Calcitonin messenger RNA encodes multiple polypeptides in a single precursor

J. W. Jacobs, Richard Goodman, W. W. Chin, P. C. Dee, J. F. Habener, N. H. Bell, J. T. Potts

Research output: Contribution to journalArticle

99 Citations (Scopus)

Abstract

Recombinant DNA techniques were used to analyze the structure of the messenger RNA encoding a precursor of calcitonin, a small calcium-regulating hormone of 32 amino acids. Analyses of the nucleotide sequences of cloned complementary DNA's comprising the entire coding sequence of the messenger RNA revealed that calcitonin is flanked at both its amino and carboxyl termini by peptide extensions linked to the hormone by short sequences of basic amino acids. The location of glycine next to the carboxyl terminal prolinamide of calcitonin is consistent with indications that glycine is required for the enzymatic amidation of proline to the prolinamide. During cellular biosynthesis, calcitonin arises from a large precursor protein by cleavages at both amino and carboxyl terminal residues of the hormone. These findings raise questions concerning the regulation of these cleavages and the potential biological functions of the precursor extensions derived from these cleavages.

Original languageEnglish (US)
Pages (from-to)457-459
Number of pages3
JournalScience
Volume213
Issue number4506
DOIs
StatePublished - 1981
Externally publishedYes

Fingerprint

Calcitonin
Messenger RNA
Peptides
Hormones
Glycine
Basic Amino Acids
Protein Precursors
Recombinant DNA
Proline
Complementary DNA
Calcium
Amino Acids
prolinamide

ASJC Scopus subject areas

  • General

Cite this

Jacobs, J. W., Goodman, R., Chin, W. W., Dee, P. C., Habener, J. F., Bell, N. H., & Potts, J. T. (1981). Calcitonin messenger RNA encodes multiple polypeptides in a single precursor. Science, 213(4506), 457-459. https://doi.org/10.1126/science.6264603

Calcitonin messenger RNA encodes multiple polypeptides in a single precursor. / Jacobs, J. W.; Goodman, Richard; Chin, W. W.; Dee, P. C.; Habener, J. F.; Bell, N. H.; Potts, J. T.

In: Science, Vol. 213, No. 4506, 1981, p. 457-459.

Research output: Contribution to journalArticle

Jacobs, JW, Goodman, R, Chin, WW, Dee, PC, Habener, JF, Bell, NH & Potts, JT 1981, 'Calcitonin messenger RNA encodes multiple polypeptides in a single precursor', Science, vol. 213, no. 4506, pp. 457-459. https://doi.org/10.1126/science.6264603
Jacobs, J. W. ; Goodman, Richard ; Chin, W. W. ; Dee, P. C. ; Habener, J. F. ; Bell, N. H. ; Potts, J. T. / Calcitonin messenger RNA encodes multiple polypeptides in a single precursor. In: Science. 1981 ; Vol. 213, No. 4506. pp. 457-459.
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AU - Bell, N. H.

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