Calcitonin gene-related peptide enhances release of native brain-derived neurotrophic factor from trigeminal ganglion neurons

Ilya Buldyrev, Nathan M. Tanner, Hui Ya Hsieh, Emily G. Dodd, Loi T. Nguyen, Agnieszka Balkowiec

Research output: Contribution to journalArticlepeer-review

91 Scopus citations


Activity-dependent plasticity in nociceptive pathways has been implicated in pathomechanisms of chronic pain syndromes. Calcitonin gene-related peptide (CGRP), which is expressed by trigeminal nociceptors, has recently been identified as a key player in the mechanism of migraine headaches. Here we show that CGRP is coexpressed with brain-derived neurotrophic factor (BDNF) in a large subset of adult rat trigeminal ganglion neurons in vivo. Using ELISA in situ, we show that CGRP (1-1000 nm) potently enhances BDNF release from cultured trigeminal neurons. The effect of CGRP is dose-dependent and abolished by pretreatment with CGRP receptor antagonist, CGRP(8-37). Intriguingly, CGRP-mediated BDNF release, unlike BDNF release evoked by physiological patterns of electrical stimulation, is independent of extracellular calcium. Depletion of intracellular calcium stores with thapsigargin blocks the CGRP-mediated BDNF release. Using transmission electron microscopy, our study also shows that BDNF-immunoreactivity is present in dense core vesicles of unmyelinated axons and axon terminals in the subnucleus caudalis of the spinal trigeminal nucleus, the primary central target of trigeminal nociceptors. Together, these results reveal a previously unknown role for CGRP in regulating BDNF availability, and point to BDNF as a candidate mediator of trigeminal nociceptive plasticity.

Original languageEnglish (US)
Pages (from-to)1338-1350
Number of pages13
JournalJournal of neurochemistry
Issue number5
StatePublished - Dec 2006


  • Brain-derived neurotrophic factor
  • Calcitonin gene-related peptide
  • Migraine
  • Pain
  • Synaptic plasticity
  • Trigeminal

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience


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