Caffeoylquinic acids in centella asiatica protect against amyloid-β toxicity

Nora E. Gray, Jeff Morré, Jeremiah Kelley, Claudia S. Maier, Jan F. Stevens, Joseph Quinn, Amala Soumyanath

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

The accumulation of amyloid-β (Aβ) is a hallmark of Alzheimer's disease and is known to result in neurotoxicity both in vivo and in vitro. We previously demonstrated that treatment with the water extract of Centella asiatica (CAW) improves learning and memory deficits in Tg2576 mice, an animal model of Aβ accumulation. However the active compounds in CAW remain unknown. Here we used two in vitro models of Aβ toxicity to confirm this neuroprotective effect and identify several active constituents of the CAW extract. CAW reduced Aβ-induced cell death and attenuated Aβ-induced changes in tau expression and phosphorylation in both the MC65 and SH-SY5Y neuroblastoma cell lines. We confirmed and quantified the presence of several mono- and dicaffeoylquinic acids (CQAs) in CAW using chromatographic separation coupled to mass spectrometry and ultraviolet spectroscopy. Multiple dicaffeoylquinic acids showed efficacy in protecting MC65 cells against Aβ-induced cytotoxicity. Isochlorogenic acid A and 1,5-dicaffeoylquinic acid were found to be the most abundant CQAs in CAW, and the most active in protecting MC65 cells from Aβ-induced cell death. Both compounds showed neuroprotective activity in MC65 and SH-SY5Y cells at concentrations comparable to their levels in CAW. Each compound not only mitigated Aβ-induced cell death, but was able to attenuate Aβ-induced alterations in tau expression and phosphorylation in both cell lines, as seen with CAW. These data suggest that CQAs are active neuroprotective components in CAW, and therefore are important markers for future studies on CAW standardization, bioavailability, and dosing.

Original languageEnglish (US)
Pages (from-to)359-373
Number of pages15
JournalJournal of Alzheimer's Disease
Volume40
Issue number2
DOIs
StatePublished - 2014

Fingerprint

Centella
Amyloid
Cell Death
Phosphorylation
Cell Line
Water Purification
Memory Disorders
Neuroprotective Agents
Neuroblastoma
Biological Availability
Mass Spectrometry
Spectrum Analysis
Alzheimer Disease
Animal Models
Learning
caffeoylquinic acid
In Vitro Techniques

Keywords

  • Amyloid-β toxicity
  • caffeoylquinic acids
  • Centella asiatica
  • neuroprotection
  • tau

ASJC Scopus subject areas

  • Psychiatry and Mental health
  • Geriatrics and Gerontology
  • Clinical Psychology
  • Medicine(all)

Cite this

Caffeoylquinic acids in centella asiatica protect against amyloid-β toxicity. / Gray, Nora E.; Morré, Jeff; Kelley, Jeremiah; Maier, Claudia S.; Stevens, Jan F.; Quinn, Joseph; Soumyanath, Amala.

In: Journal of Alzheimer's Disease, Vol. 40, No. 2, 2014, p. 359-373.

Research output: Contribution to journalArticle

Gray, Nora E. ; Morré, Jeff ; Kelley, Jeremiah ; Maier, Claudia S. ; Stevens, Jan F. ; Quinn, Joseph ; Soumyanath, Amala. / Caffeoylquinic acids in centella asiatica protect against amyloid-β toxicity. In: Journal of Alzheimer's Disease. 2014 ; Vol. 40, No. 2. pp. 359-373.
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