Caenorhabditis elegans innate immune response triggered by Salmonella enterica requires intact LPS and is mediated by a MAPK signaling pathway

Alejandro Aballay, Eliana Drenkard, Layla R. Hilbun, Frederick M. Ausubel

Research output: Contribution to journalArticle

120 Citations (Scopus)

Abstract

Compared to mammals, insects, and plants, relatively little is known about innate immune responses in the nematode Caenorhabditis elegans. Previous work showed that Salmonella enterica serovars cause a persistent infection in the C. elegans intestine [1, 2] that triggers gonadal programmed cell death (PCD) and that C. elegans cell death (ced) mutants are more susceptible to Salmonella-mediated killing [3]. To further dissect the role of PCD in C. elegans innate immunity, we identified both C. elegans and S. enterica factors that affect the elicitation of Salmonella-induced PCD. Salmonella-elicited PCD was shown to require the C. elegans homolog of the mammalian p38 mitogen-activated protein kinase (MAPK) encoded by the pmk-1 gene. Inactivation of pmk-1 by RNAi blocked Salmonella-elicited PCD, and epistasis analysis showed that CED-9 lies downstream of PMK-1. Wild-type Salmonella lipopolysaccharide (LPS) was also shown to be required for the elicitation of PCD, as well as for persistence of Salmonella in the C. elegans intestine. However, a presumptive C. elegans TOLL signaling pathway did not appear to be required for the PCD response to Salmonella. These results establish a PMK-1-dependant PCD pathway as a C. elegans innate immune response to Salmonella.

Original languageEnglish (US)
Pages (from-to)47-52
Number of pages6
JournalCurrent Biology
Volume13
Issue number1
DOIs
StatePublished - Jan 8 2003
Externally publishedYes

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Salmonella
Salmonella enterica
Caenorhabditis elegans
Cell death
Mitogen-Activated Protein Kinases
mitogen-activated protein kinase
Innate Immunity
lipopolysaccharides
Lipopolysaccharides
Cell Death
apoptosis
Intestines
intestines
innate immunity
Mammals
epistasis
p38 Mitogen-Activated Protein Kinases
RNA Interference
Insects
cell death

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Caenorhabditis elegans innate immune response triggered by Salmonella enterica requires intact LPS and is mediated by a MAPK signaling pathway. / Aballay, Alejandro; Drenkard, Eliana; Hilbun, Layla R.; Ausubel, Frederick M.

In: Current Biology, Vol. 13, No. 1, 08.01.2003, p. 47-52.

Research output: Contribution to journalArticle

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