Ca2+/Calmodulin-dependent Protein Kinase IV Stimulates Nuclear Factor-κB Transactivation via Phosphorylation of the p65 Subunit

Moon Kyoo Jang, Young Hwa Goo, Young Chang Sohn, Yun Sung Kim, Soo-Kyung Lee, Heonjoong Kang, JaeHun Cheong, Jae Lee

Research output: Contribution to journalArticle

73 Citations (Scopus)

Abstract

Calmodulin-dependent protein kinase IV (CaMKIV) is a key mediator of Ca2+-induced gene expression. In this study, CaMKIV was found to directly associate with and phosphorylate the nuclear factor-κB (NFκB) component p65 both in vitro and in vivo. The phosphorylation of p65 by CaMKIV resulted in recruitment of transcription coactivator cAMP-response element-binding protein-binding protein and concomitant release of corepressor silencing mediator for retinoid and thyroid hormone receptors, as demonstrated by the glutathione S-transferase pull down and mammalian two hybrid assays. In addition, cotransfection of CaMKIV resulted in cytosolic translocation of the silencing mediator for retinoid and thyroid hormone receptors. Consistent with these results, cotransfected CaMKIV dramatically stimulated the NFκB transactivation in mammalian cells. From these results, NFκB is suggested to be a novel downstream effector molecule of CaMKIV.

Original languageEnglish (US)
Pages (from-to)20005-20010
Number of pages6
JournalJournal of Biological Chemistry
Volume276
Issue number23
DOIs
StatePublished - Jun 8 2001
Externally publishedYes

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Calcium-Calmodulin-Dependent Protein Kinase Type 4
Phosphorylation
Transcriptional Activation
Thyroid Hormone Receptors
Retinoids
Cyclic AMP Response Element-Binding Protein
Co-Repressor Proteins
Two-Hybrid System Techniques
Transcription
Glutathione Transferase
Protein Binding
Gene expression
Assays
Carrier Proteins
Cells
Gene Expression
Molecules

ASJC Scopus subject areas

  • Biochemistry

Cite this

Ca2+/Calmodulin-dependent Protein Kinase IV Stimulates Nuclear Factor-κB Transactivation via Phosphorylation of the p65 Subunit. / Jang, Moon Kyoo; Goo, Young Hwa; Sohn, Young Chang; Kim, Yun Sung; Lee, Soo-Kyung; Kang, Heonjoong; Cheong, JaeHun; Lee, Jae.

In: Journal of Biological Chemistry, Vol. 276, No. 23, 08.06.2001, p. 20005-20010.

Research output: Contribution to journalArticle

Jang, Moon Kyoo ; Goo, Young Hwa ; Sohn, Young Chang ; Kim, Yun Sung ; Lee, Soo-Kyung ; Kang, Heonjoong ; Cheong, JaeHun ; Lee, Jae. / Ca2+/Calmodulin-dependent Protein Kinase IV Stimulates Nuclear Factor-κB Transactivation via Phosphorylation of the p65 Subunit. In: Journal of Biological Chemistry. 2001 ; Vol. 276, No. 23. pp. 20005-20010.
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AU - Sohn, Young Chang

AU - Kim, Yun Sung

AU - Lee, Soo-Kyung

AU - Kang, Heonjoong

AU - Cheong, JaeHun

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