Ca2+-triggers massive exocytosis in Chinese hamster ovary cells

J. R. Coorssen, H. Schmitt, Wolfhard Almers

Research output: Contribution to journalArticle

109 Citations (Scopus)

Abstract

We have tracked the cell surface area of CHO cells by measuring the membrane capacitance, C(m). An increase in cytosolic [Ca2+], [Ca2+](i), increased the cell surface area by 20-30%. At micromolar [Ca2+](i) the increase occurred in minutes, while at 20 μM or higher [Ca2+](i) it occurred in seconds and was transient. GTPγS caused a 3% increase even at 0.1 μM [Ca2+](i). We conclude that CHO cells, previously thought capable only of constitutive exocytosis, can perform Ca2+-triggered exocytosis that is both massive and rapid. Ca2+-triggered exocytosis was also observed in 3T3 fibroblasts. Our findings add evidence to the view that Ca induces exocytosis in cells other than known secretory cells.

Original languageEnglish (US)
Pages (from-to)3787-3791
Number of pages5
JournalEMBO Journal
Volume15
Issue number15
StatePublished - Aug 1 1996
Externally publishedYes

Fingerprint

Exocytosis
Cricetulus
Ovary
Cells
CHO Cells
Fibroblasts
Capacitance
Membranes
Cell Membrane

Keywords

  • 3T3 fibroblasts
  • Endocytosis
  • Flash photolysis
  • GTPγS
  • Plasmalemmal repair

ASJC Scopus subject areas

  • Cell Biology
  • Genetics

Cite this

Coorssen, J. R., Schmitt, H., & Almers, W. (1996). Ca2+-triggers massive exocytosis in Chinese hamster ovary cells. EMBO Journal, 15(15), 3787-3791.

Ca2+-triggers massive exocytosis in Chinese hamster ovary cells. / Coorssen, J. R.; Schmitt, H.; Almers, Wolfhard.

In: EMBO Journal, Vol. 15, No. 15, 01.08.1996, p. 3787-3791.

Research output: Contribution to journalArticle

Coorssen, JR, Schmitt, H & Almers, W 1996, 'Ca2+-triggers massive exocytosis in Chinese hamster ovary cells', EMBO Journal, vol. 15, no. 15, pp. 3787-3791.
Coorssen JR, Schmitt H, Almers W. Ca2+-triggers massive exocytosis in Chinese hamster ovary cells. EMBO Journal. 1996 Aug 1;15(15):3787-3791.
Coorssen, J. R. ; Schmitt, H. ; Almers, Wolfhard. / Ca2+-triggers massive exocytosis in Chinese hamster ovary cells. In: EMBO Journal. 1996 ; Vol. 15, No. 15. pp. 3787-3791.
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