Ca2+-dependent enhancement of release by subthreshold somatic depolarization

Jason M. Christie, Delia N. Chiu, Craig Jahr

Research output: Contribution to journalArticle

70 Citations (Scopus)

Abstract

In many neurons, subthreshold somatic depolarization can spread electrotonically into the axon and modulate subsequent spike-evoked transmission. Although release probability is regulated by intracellular Ca 2+, the Ca2+ dependence of this modulatory mechanism has been debated. Using paired recordings from synaptically connected molecular layer interneurons (MLIs) of the rat cerebellum, we observed Ca 2+-mediated strengthening of release following brief subthreshold depolarization of the soma. Two-photon microscopy revealed that, at the axon, somatic depolarization evoked Ca2+ influx through voltage-sensitive Ca2+ channels and facilitated spike-evoked Ca2+ entry. Exogenous Ca2+ buffering diminished these Ca2+ transients and eliminated the strengthening of release. Axonal Ca2+ entry elicited by subthreshold somatic depolarization also triggered asynchronous transmission that may deplete vesicle availability and thereby temper release strengthening. In this cerebellar circuit, activity-dependent presynaptic plasticity depends on Ca2+ elevations resulting from both sub- and suprathreshold electrical activity initiated at the soma.

Original languageEnglish (US)
Pages (from-to)62-69
Number of pages8
JournalNature Neuroscience
Volume14
Issue number1
DOIs
StatePublished - Jan 2011

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Carisoprodol
Axons
Interneurons
Photons
Cerebellum
Microscopy
Neurons

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  • Neuroscience(all)

Cite this

Ca2+-dependent enhancement of release by subthreshold somatic depolarization. / Christie, Jason M.; Chiu, Delia N.; Jahr, Craig.

In: Nature Neuroscience, Vol. 14, No. 1, 01.2011, p. 62-69.

Research output: Contribution to journalArticle

Christie, Jason M. ; Chiu, Delia N. ; Jahr, Craig. / Ca2+-dependent enhancement of release by subthreshold somatic depolarization. In: Nature Neuroscience. 2011 ; Vol. 14, No. 1. pp. 62-69.
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