Ca2+-activated, large conductance K+ channel in the ovary: Identification, characterization, and functional involvement in steroidogenesis

Lars Kunz, Andrea Thalhammer, Frank D. Berg, Ulrike Berg, Diane M. Duffy, Richard Stouffer, Gregory Dissen, Sergio Ojeda, Artur Mayerhofer

    Research output: Contribution to journalArticle

    36 Citations (Scopus)

    Abstract

    Progesterone production by the corpus luteum is a process vital for reproduction. In humans its secretion is stimulated by the placental hormone human chorionic gonadotropin (hCG), and this stimulatory action can also be observed in cultured human luteinized granulosa cells (GCs). We now provide evidence that opening of a Ca2+-activated K+ channel, the BKCa, is crucially involved in this process. Immunohistochemistry and RT-PCR revealed the presence of the pore-forming α-subunit in human luteinized GCs and in luteal cells of human, macaque, and rat, implying that BKCa channels are important throughout species. Blocking of BKCa channels by iberiotoxin attenuated hCG-induced progesterone secretion. The inhibitory action of iberiotoxin suggests that BKCa channels are activated in the course of hCG-induced steroido-genesis. In search of physiological activators we used an electrophysiological approach and could preclude a direct regulation of channel activity by hCG or GC-derived steroids (progesterone and 17β-estradiol). Instead, the peptide hormone oxytocin and an acetylcholine (ACh) agonist, carbachol, evoked transient BKCa currents and membrane hyperpolarization. These two molecules are both secreted by GCs and act via raised intracellular Ca2+ levels. The release of oxytocin is stimulated by hCG, and a similar mechanism is likely in the case of ACh. We conclude that BKCa channel activity in GCs is mediated by components of the intraovarian signaling system, thereby interlinking a systemic hormonal and a local neuroendocrine system in control of steroidogenesis.

    Original languageEnglish (US)
    Pages (from-to)5566-5574
    Number of pages9
    JournalJournal of Clinical Endocrinology and Metabolism
    Volume87
    Issue number12
    DOIs
    StatePublished - Dec 1 2002

    Fingerprint

    Granulosa Cells
    Chorionic Gonadotropin
    Ovary
    Progesterone
    Oxytocin
    Placental Hormones
    Luteal Cells
    Calcium-Activated Potassium Channels
    Cholinergic Agonists
    Neurosecretory Systems
    Peptide Hormones
    Corpus Luteum
    Macaca
    Carbachol
    Acetylcholine
    Reproduction
    Rats
    Estradiol
    Immunohistochemistry
    Steroids

    ASJC Scopus subject areas

    • Biochemistry
    • Endocrinology, Diabetes and Metabolism

    Cite this

    Ca2+-activated, large conductance K+ channel in the ovary : Identification, characterization, and functional involvement in steroidogenesis. / Kunz, Lars; Thalhammer, Andrea; Berg, Frank D.; Berg, Ulrike; Duffy, Diane M.; Stouffer, Richard; Dissen, Gregory; Ojeda, Sergio; Mayerhofer, Artur.

    In: Journal of Clinical Endocrinology and Metabolism, Vol. 87, No. 12, 01.12.2002, p. 5566-5574.

    Research output: Contribution to journalArticle

    Kunz, Lars ; Thalhammer, Andrea ; Berg, Frank D. ; Berg, Ulrike ; Duffy, Diane M. ; Stouffer, Richard ; Dissen, Gregory ; Ojeda, Sergio ; Mayerhofer, Artur. / Ca2+-activated, large conductance K+ channel in the ovary : Identification, characterization, and functional involvement in steroidogenesis. In: Journal of Clinical Endocrinology and Metabolism. 2002 ; Vol. 87, No. 12. pp. 5566-5574.
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    AU - Duffy, Diane M.

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