C1 adrenergic neurons are contacted by presynaptic profiles containing delta-opioid receptor immunoreactivity

T. A. Milner, C. T. Drake, Sue Aicher

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Ligands of the δ-opioid receptor tonically influence sympathetic outflow. Some of the actions of δ-opioid receptor agonists may be mediated through C1 adrenergic neurons in the rostral ventrolateral medulla. The goal of this study was to determine whether C1 adrenergic neurons or their afferents contain δ-opioid receptors. Single sections through the rostral ventrolateral medulla were labeled for δ-opioid receptor using the immunoperoxidase method and the epinephrine synthesizing enzyme phenylethanolamine N-methyltransferase (PNMT) using the immunogold method, and examined at the light and electron microscopic level. Few (∼5% of 903) profiles dually labeled for PNMT and δ-opioid receptor were detected; most of these were dendrites with diameters 50% of 250) of δ-opioid receptor-immunoreactive axons and axon terminals contacted PNMT-immunoreactive profiles. Most of the contacts formed by δ-opioid receptor-immunoreactive profiles (∼75% of 132) were on single-labeled PNMT-immunoreactive dendrites with diameters

Original languageEnglish (US)
Pages (from-to)691-701
Number of pages11
JournalNeuroscience
Volume110
Issue number4
DOIs
StatePublished - Apr 3 2002

Fingerprint

Adrenergic Neurons
delta Opioid Receptor
Opioid Receptors
Phenylethanolamine N-Methyltransferase
Dendrites
Presynaptic Terminals
Epinephrine
Axons
Electrons
Ligands
Light
Enzymes

Keywords

  • Electron microscopy
  • Enkephalin
  • Opioid peptides
  • Reticulospinal neurons
  • Rostral ventrolateral medulla
  • Ultrastructure

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

C1 adrenergic neurons are contacted by presynaptic profiles containing delta-opioid receptor immunoreactivity. / Milner, T. A.; Drake, C. T.; Aicher, Sue.

In: Neuroscience, Vol. 110, No. 4, 03.04.2002, p. 691-701.

Research output: Contribution to journalArticle

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