TY - JOUR
T1 - Butanediol induced ketosis increases tolerance to hypoxia in the mouse
AU - Kirsch, Jeffrey R.
AU - D’Alecy, Louis G.
AU - Moncroo, Peter B.
N1 - Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.
PY - 1980
Y1 - 1980
N2 - In previous studies from our laboratory a positive correlation between elevated blood ketone levels and the survival time (ST) during hypoxia (4-5% oxygen) was observed in fasted and alloxan diabetic mice. To test the hypothesis that ketosis was somehow increasing the tolerance of mice to hypoxia, we induced ketosis by either oral (PO), intraperitoneal (IP), or intravenous (IV) 1,3-butanediol (BD). Blood betahydroxybutyrate increased from 033 ± 0.06 mM to 3.32 ± 0.08 mM for PO, 1.2 ± 0.2 mM for IV and 0.83 ± 0.15 mM for IP. BD was associated with an increase in ST to 458% (n = 19) when given PO, 217% (n = 12) by IP route, and 560% (n = 13) by the IV route. The effect of ambient temperature (Ta) on this phenomenon was evaluated at 12, 22, 32, and 34° C. At each TaIV BD at 1.4 mmole/mouse was associated with an increase in ST to 525,559,151, and 145% of control, respectively. The absolute ST of both control and treated mice was greater at Ta of 12 and 22°C than at 32 and 34°C. Rectal temperature was continuously recorded and no differences were detected between control and treated groups just prior to hypoxia except for the group treated at Ta of 12°C. Hypoxia, however, was associated with a decrease in body temperature in each group. It is concluded that the artificial induction of ketosis by BD is associated with an increase in ST of mice exposed to hypoxia.
AB - In previous studies from our laboratory a positive correlation between elevated blood ketone levels and the survival time (ST) during hypoxia (4-5% oxygen) was observed in fasted and alloxan diabetic mice. To test the hypothesis that ketosis was somehow increasing the tolerance of mice to hypoxia, we induced ketosis by either oral (PO), intraperitoneal (IP), or intravenous (IV) 1,3-butanediol (BD). Blood betahydroxybutyrate increased from 033 ± 0.06 mM to 3.32 ± 0.08 mM for PO, 1.2 ± 0.2 mM for IV and 0.83 ± 0.15 mM for IP. BD was associated with an increase in ST to 458% (n = 19) when given PO, 217% (n = 12) by IP route, and 560% (n = 13) by the IV route. The effect of ambient temperature (Ta) on this phenomenon was evaluated at 12, 22, 32, and 34° C. At each TaIV BD at 1.4 mmole/mouse was associated with an increase in ST to 525,559,151, and 145% of control, respectively. The absolute ST of both control and treated mice was greater at Ta of 12 and 22°C than at 32 and 34°C. Rectal temperature was continuously recorded and no differences were detected between control and treated groups just prior to hypoxia except for the group treated at Ta of 12°C. Hypoxia, however, was associated with a decrease in body temperature in each group. It is concluded that the artificial induction of ketosis by BD is associated with an increase in ST of mice exposed to hypoxia.
UR - http://www.scopus.com/inward/record.url?scp=0018970443&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0018970443&partnerID=8YFLogxK
U2 - 10.1161/01.STR.11.5.506
DO - 10.1161/01.STR.11.5.506
M3 - Article
C2 - 6775394
AN - SCOPUS:0018970443
VL - 11
SP - 506
EP - 513
JO - Stroke
JF - Stroke
SN - 0039-2499
IS - 5
ER -