Bright AIE Nanoparticles with F127 Encapsulation for Deep-Tissue Three-Photon Intravital Brain Angiography

Yalun Wang, Xiao Han, Wang Xi, Jinyu Li, Anna Roe, Ping Lu, Jun Qian

Research output: Contribution to journalArticle

19 Scopus citations

Abstract

Deep-tissue imaging is of great significance to biological applications. In this paper, a deep-red emissive luminogen 2,3-bis(4′-(diphenylamino)-[1,1′-biphenyl]-4-yl) fumaronitrile (TPATCN) with aggregation-induced emission (AIE) feature is prepared. TPATCN molecules were then encapsulated within a polymeric matrix of Pluronic F-127 to form nanoparticles (NPs). TPATCN NPs exhibit bright three-photon fluorescence (3PF) in deep-red region, together with high chemical stability, good photostability, and biocompatibility. They are further utilized for in vivo 3PF imaging of the brain vasculature of mice, under the excitation of a 1550 nm femtosecond laser. A vivid 3D reconstruction of the brain vasculature is then built with a penetration depth of 875 µm, which is the largest in ever reported 3PF imaging based on AIE NPs. After that, by collecting both of the 3PF and third-harmonic generation signals, multichannel nonlinear optical imaging of the brain blood vessels is further realized. These results will be helpful to study the structures and functions of the brain in the future.

Original languageEnglish (US)
Article number1700685
JournalAdvanced healthcare materials
Volume6
Issue number21
DOIs
StatePublished - Nov 8 2017
Externally publishedYes

Keywords

  • aggregation-induced emission (AIE)
  • deep-tissue imaging
  • F127 encapsulation
  • in vivo
  • three-photon fluorescence (3PF)

ASJC Scopus subject areas

  • Biomaterials
  • Biomedical Engineering
  • Pharmaceutical Science

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