Brain-specific protein C activation during carotid artery occlusion in humans

Richard F. Macko, Lois A. Killewich, José A. Fernández, D. Kim Cox, Andras Gruber, John H. Griffin

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Background and Purpose - Activation of plasma protein C (PC) zymogen by thrombin-thrombomodulin at the endothelial surface is an important endogenous antithrombotic mechanism. It is unknown whether activated protein C (APC) is generated in vivo in the cerebrovasculature, because there is only limited thrombomodulin expression in human brain vascular endothelium. Therefore, we tested the hypothesis that carotid occlusion produces brain-specific PC activation. Methods - Blood samples were simultaneously collected from the ipsilateral internal jugular vein and radial artery before and during carotid cross-clamping and on 'de-occlusion' in 8 awake patients undergoing routine carotid endarterectomy. Plasma PC zymogen and circulating APC levels were measured using enzyme immunocapture assay and expressed as percent of pooled plasma controls. Results - Internal jugular vein APC levels increased 28% exclusively during carotid occlusion and then decreased 32% with de-occlusion (F=8.1, P

Original languageEnglish (US)
Pages (from-to)542-545
Number of pages4
JournalStroke
Volume30
Issue number3
StatePublished - Mar 1999
Externally publishedYes

Fingerprint

Protein C
Carotid Arteries
Brain
Thrombomodulin
Enzyme Precursors
Jugular Veins
Blood Proteins
Radial Artery
Carotid Endarterectomy
Vascular Endothelium
Enzyme Assays
Constriction
Thrombin

Keywords

  • Cerebral ischemia
  • Protein C
  • Stroke
  • Thrombomodulin

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Neuroscience(all)

Cite this

Macko, R. F., Killewich, L. A., Fernández, J. A., Cox, D. K., Gruber, A., & Griffin, J. H. (1999). Brain-specific protein C activation during carotid artery occlusion in humans. Stroke, 30(3), 542-545.

Brain-specific protein C activation during carotid artery occlusion in humans. / Macko, Richard F.; Killewich, Lois A.; Fernández, José A.; Cox, D. Kim; Gruber, Andras; Griffin, John H.

In: Stroke, Vol. 30, No. 3, 03.1999, p. 542-545.

Research output: Contribution to journalArticle

Macko, RF, Killewich, LA, Fernández, JA, Cox, DK, Gruber, A & Griffin, JH 1999, 'Brain-specific protein C activation during carotid artery occlusion in humans', Stroke, vol. 30, no. 3, pp. 542-545.
Macko RF, Killewich LA, Fernández JA, Cox DK, Gruber A, Griffin JH. Brain-specific protein C activation during carotid artery occlusion in humans. Stroke. 1999 Mar;30(3):542-545.
Macko, Richard F. ; Killewich, Lois A. ; Fernández, José A. ; Cox, D. Kim ; Gruber, Andras ; Griffin, John H. / Brain-specific protein C activation during carotid artery occlusion in humans. In: Stroke. 1999 ; Vol. 30, No. 3. pp. 542-545.
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