Brain expression and song regulation of the cholecystokinin gene in the zebra finch (Taeniopygia guttata)

Peter V. Lovell, Claudio Mello

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

The gene encoding cholecystokinin (Cck) is abundantly expressed in the mammalian brain and has been associated with such functions as feeding termination and satiety, locomotion and self-stimulation, the modulation of anxiety-like behaviors, and learning and memory. Here we describe the brain expression and song regulation of Cck in the brain of the adult male zebra finch (Taeniopygia guttata), a songbird species. Using in situ hybridization we demonstrate that Cck is highly expressed in several discrete brain regions, most prominently the caudalmost portion of the hippocampal formation, the caudodorsal nidopallial shelf and the caudomedial nidopallium (NCM), the core or shell regions of dorsal thalamic nuclei, dopaminergic cell groups in the mesencephalon and pons, the principal nucleus of the trigeminal nerve, and the dorsal raphe. Cck was largely absent in song control system, a group of nuclei required for vocal learning and song production in songbirds, although sparse labeling was detected throughout the striatum, including song nucleus area X. We also show that levels of Cck mRNA and the number of labeled cells increase in the NCM of males and females following auditory stimulation with conspecific song. Double labeling further reveals that the majority of Cck cells, excluding those in the reticular nucleus of the thalamus, are non-GABAergic. Together, these data provide the first comprehensive characterization of Cck expression in a songbird, and suggest a possible involvement of Cck regulation in important aspects of birdsong biology, such as perceptual processing, auditory memorization, and/or vocal-motor control of song production.

Original languageEnglish (US)
Pages (from-to)211-237
Number of pages27
JournalJournal of Comparative Neurology
Volume519
Issue number2
DOIs
StatePublished - Feb 1 2011

Fingerprint

Finches
Equidae
Cholecystokinin
Music
Brain
Genes
Songbirds
Learning
Self Stimulation
Acoustic Stimulation
Thalamic Nuclei
Trigeminal Nerve
Pons
Locomotion
Mesencephalon
Thalamus
In Situ Hybridization
Hippocampus
Anxiety
Cell Count

Keywords

  • Auditory
  • Gene regulation
  • Song learning
  • Songbird
  • Vocal control system

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Brain expression and song regulation of the cholecystokinin gene in the zebra finch (Taeniopygia guttata). / Lovell, Peter V.; Mello, Claudio.

In: Journal of Comparative Neurology, Vol. 519, No. 2, 01.02.2011, p. 211-237.

Research output: Contribution to journalArticle

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