Brain-derived neurotrophic factor rapidly increases AMPA receptor surface expression in rat nucleus accumbens

Xuan Li, Marina Wolf

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

In the rodent nucleus accumbens (NAc), cocaine elevates levels of brain-derived neurotrophic factor (BDNF). Conversely, BDNF can augment cocaine-related behavioral responses. The latter could reflect enhancement of α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor (AMPAR) transmission, because AMPARs in the NAc mediate some cocaine-induced behaviors. Furthermore, in vitro studies in other cell types show that BDNF can promote AMPAR synaptic delivery. In this study, we investigated whether BDNF similarly promotes AMPAR trafficking in the adult rat NAc. After unilateral intracranial injection of BDNF into NAc core or shell, rats were killed at post-injection times ranging from 30min to 3days. NAc core or shell tissue from both injected and non-injected hemispheres was analysed by Western blotting. A protein cross-linking assay was used to measure AMPAR surface expression. Assessment of tropomyosin receptor kinase B signaling demonstrated that injected BDNF was biologically active. BDNF injection into NAc core, but not NAc shell, led to a protein synthesis- and extracellular signal-regulated kinase-dependent increase in cell surface GluA1 and a trend towards increased total GluA1. This was detected 30min post-injection but not at longer time-points. GluA2 and GluA3 were unaffected, suggesting an effect of BDNF on homomeric GluA1 Ca 2+-permeable AMPARs. These results demonstrate that exogenous BDNF rapidly increases AMPAR surface expression in the rat NAc core, raising the possibility of a relationship between increases in endogenous BDNF levels and alterations in AMPAR transmission observed in the NAc of cocaine-experienced rats.

Original languageEnglish (US)
Pages (from-to)190-198
Number of pages9
JournalEuropean Journal of Neuroscience
Volume34
Issue number2
DOIs
StatePublished - Jul 1 2011
Externally publishedYes

Fingerprint

AMPA Receptors
Brain-Derived Neurotrophic Factor
Nucleus Accumbens
Cocaine
Injections
Extracellular Signal-Regulated MAP Kinases
Rodentia
Proteins
Western Blotting
Acids

Keywords

  • Ca -permeable AMPA receptor
  • Cocaine
  • ERK
  • TrkB

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Brain-derived neurotrophic factor rapidly increases AMPA receptor surface expression in rat nucleus accumbens. / Li, Xuan; Wolf, Marina.

In: European Journal of Neuroscience, Vol. 34, No. 2, 01.07.2011, p. 190-198.

Research output: Contribution to journalArticle

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