Brain-derived neurotrophic factor

A novel human ovarian follicular protein

David Seifer, Bo Feng, Robert M. Shelden, Shiling Chen, Cheryl F. Dreyfus

Research output: Contribution to journalArticle

77 Citations (Scopus)

Abstract

Neurotrophins are a family of soluble polypeptide growth factors widely recognized for their roles in the mammalian nervous system. One such neurotrophin, brain-derived neurotrophic factor (BDNF) was originally described in the nervous system but has now been shown to be expressed in a variety of nonneuronal tissues including endocrine tissues. We examined the human ovarian follicle for its possible secretion of BDNF and further studied mouse oocytes to determine BDNF's possible influence upon oocyte maturation. In a series of experiments derived from human specimens from in vitro fertilization following oocyte retrieval, BDNF was detected in human follicular fluid. To define the source of BDNF, cumulus granulosa cells (the cells that immediately surround the developing oocyte) were grown in cell culture for 1-2 d. BDNF protein increased over 24 h in the culture medium. Moreover, the release of BDNF was enhanced upon stimulation with cAMP or forskolin, an activator of cAMP. In contrast, mural granulosa (cells lining the follicle), oocytes, and embryos did not release appreciable quantities of BDNF. To examine possible targets of BDNF, mouse studies were used to localize the BDNF receptor, Trk B, immunocytochemically. The receptor was present on the surface of isolated oocytes. Moreover, BDNF promoted mouse oocyte maturation in culture. These experiments demonstrate for the first time the presence and secretion of BDNF from follicular cells in the human ovary and suggest a possible role for BDNF in the regulation and modulation of oocyte maturation.

Original languageEnglish (US)
Pages (from-to)655-659
Number of pages5
JournalJournal of Clinical Endocrinology and Metabolism
Volume87
Issue number2
DOIs
StatePublished - 2002
Externally publishedYes

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Brain-Derived Neurotrophic Factor
Oocytes
Proteins
Nerve Growth Factors
Neurology
Nervous System
trkB Receptor
Tissue
Cumulus Cells
Oocyte Retrieval
Follicular Fluid
Ovarian Follicle
Granulosa Cells
Colforsin
Fertilization in Vitro
Linings
Cell culture
Culture Media
Ovary
Intercellular Signaling Peptides and Proteins

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology, Diabetes and Metabolism

Cite this

Brain-derived neurotrophic factor : A novel human ovarian follicular protein. / Seifer, David; Feng, Bo; Shelden, Robert M.; Chen, Shiling; Dreyfus, Cheryl F.

In: Journal of Clinical Endocrinology and Metabolism, Vol. 87, No. 2, 2002, p. 655-659.

Research output: Contribution to journalArticle

Seifer, David ; Feng, Bo ; Shelden, Robert M. ; Chen, Shiling ; Dreyfus, Cheryl F. / Brain-derived neurotrophic factor : A novel human ovarian follicular protein. In: Journal of Clinical Endocrinology and Metabolism. 2002 ; Vol. 87, No. 2. pp. 655-659.
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