Bovine sperm forward motility protein. Partial purification and characterization

Ted Acott, D. D. Hoskins

Research output: Contribution to journalArticle

81 Citations (Scopus)

Abstract

A protein, present in bovine seminal plasma, initiates forward motility in immature, immotile caput spermatozoa that have been incubated with a cyclic AMP phosphodiesterase inhibitor. An improved motility assay was developed to study this process and the protein involved. This forward motility protein exhibits multiple forms when fractionated on the basis of charge or molecular weight. Molecular sieving in urea or sodium dodecyl sulfate and dithiothreitol results in a single peak of activity which will re-form the larger aggregates in the absence of these agents. The molecular weight of this monomeric motility protein, as estimated from molecular sieving under these dissociating conditions, is 37,500. The forward motility protein can be partially purified by heat treatment, gel chromatography in urea, and affinity chromatography on concanavalin A/agarose. Enzymatic treatments further suggest a glycoprotein nature, i.e. treatment with β-galactosidase, neuraminidase, α-mannosidase, or galactose oxidase reduces its activity by 50%; treatment with trypsin completely abolishes forward motility protein activity. On the basis of concurrent studies on the activity, properties, and distribution of forward motility protein in bovine body fluids, it is suggested that this protein is involved in the development of the capacity for motility as sperm traverse the epididymis.

Original languageEnglish (US)
Pages (from-to)6744-6750
Number of pages7
JournalJournal of Biological Chemistry
Volume253
Issue number19
StatePublished - 1978

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Molecular Motor Proteins
Sperm Motility
Purification
Urea
Molecular Weight
Molecular weight
Mannosidases
Galactose Oxidase
Galactosidases
Affinity chromatography
Proteins
Phosphodiesterase Inhibitors
Epididymis
Dithiothreitol
Body fluids
Neuraminidase
Body Fluids
Concanavalin A
Chromatography
Semen

ASJC Scopus subject areas

  • Biochemistry

Cite this

Bovine sperm forward motility protein. Partial purification and characterization. / Acott, Ted; Hoskins, D. D.

In: Journal of Biological Chemistry, Vol. 253, No. 19, 1978, p. 6744-6750.

Research output: Contribution to journalArticle

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