Bovine sperm forward motility protein: Binding to epididymal spermatozoa

Ted Acott, D. D. Hoskins

Research output: Contribution to journalArticle

66 Citations (Scopus)

Abstract

Immature spermatozoa from the bovine caput epididymis develop an uncoordinated thrashing motion in response to an elevation in cyclic AMP levels and convert to strong progressive motility upon the subsequent addition of bovine forward motility protein. We show that this protein activates caput sperm in a switchlike, all-or-none manner and that the activation is not easily reversible. We also present evidence that the forward motility protein binds to caput sperm as they pass through the epididymis. First, the continued presence of free forward motility protein is not necessary. Second, the activation does not involve a simple enzymatic mechanism. Third, the forward motility protein can be depleted from solution by caput, but not caudal, epididymal sperm. Fourth, forward motility protein can be extracted from caudal, but not caput, epididymal sperm with Triton X-100.

Original languageEnglish (US)
Pages (from-to)234-240
Number of pages7
JournalBiology of Reproduction
Volume24
Issue number2
StatePublished - 1981

Fingerprint

Molecular Motor Proteins
Sperm Motility
Protein Binding
Spermatozoa
Epididymis
Octoxynol
Cyclic AMP
Proteins

ASJC Scopus subject areas

  • Cell Biology
  • Developmental Biology
  • Embryology

Cite this

Bovine sperm forward motility protein : Binding to epididymal spermatozoa. / Acott, Ted; Hoskins, D. D.

In: Biology of Reproduction, Vol. 24, No. 2, 1981, p. 234-240.

Research output: Contribution to journalArticle

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