Bovine sperm forward motility protein: Binding to epididymal spermatozoa

T. S. Acott, D. D. Hoskins

Research output: Contribution to journalArticle

66 Scopus citations

Abstract

Immature spermatozoa from the bovine caput epididymis develop an uncoordinated thrashing motion in response to an elevation in cyclic AMP levels and convert to strong progressive motility upon the subsequent addition of bovine forward motility protein. We show that this protein activates caput sperm in a switchlike, all-or-none manner and that the activation is not easily reversible. We also present evidence that the forward motility protein binds to caput sperm as they pass through the epididymis. First, the continued presence of free forward motility protein is not necessary. Second, the activation does not involve a simple enzymatic mechanism. Third, the forward motility protein can be depleted from solution by caput, but not caudal, epididymal sperm. Fourth, forward motility protein can be extracted from caudal, but not caput, epididymal sperm with Triton X-100.

Original languageEnglish (US)
Pages (from-to)234-240
Number of pages7
JournalBiology of reproduction
Volume24
Issue number2
DOIs
StatePublished - Jan 1 1981

ASJC Scopus subject areas

  • Reproductive Medicine
  • Cell Biology

Fingerprint Dive into the research topics of 'Bovine sperm forward motility protein: Binding to epididymal spermatozoa'. Together they form a unique fingerprint.

  • Cite this