Bone morphogenetic protein-2-impregnated biomimetic scaffolds successfully induce bone healing in a marginal mandibular defect

Adam S. Deconde, Douglas Sidell, Min Lee, Olga Bezouglaia, Kyle Low, David Elashoff, Tristan Grogan, Sotirios Tetradis, Tara Aghaloo, Maie St. John

Research output: Contribution to journalArticle

13 Scopus citations

Abstract

Objectives/Hypothesis To test the osteoregenerative potential and dosing of bone morphogenetic protein-2 (BMP-2)-impregnated biomimetic scaffolds in a rat model of a mandibular defect. Study Design Prospective study using an animal model. Methods Varied doses of BMP-2 (0.5, 1, 0.5, 0.5 in microspheres, 5, and 15 μg) were absorbed onto a biomimetic scaffold. Scaffolds were then implanted into marginal mandibular defects in rats. Blank scaffolds and unfilled defects were used as negative controls. Two months postoperatively, bone healing was analyzed with microcomputerized tomography (microCT). Results MicroCT analysis demonstrated that all doses of BMP-2 induced successful healing of marginal mandibular defects in a rat mandible. Increasing doses of BMP-2 on the scaffolds produced increased tissue healing, with 15 μg demonstrating significantly more healing than all other dosing (P <.01). Conclusions BMP-2-impregnated biomimetic scaffolds successfully induce bone healing in a marginal mandibular defect in the rat. Percentage healing of defect, percentage of bone within healed tissue, and total bone volume are all a function of BMP-2 dosing. There appears to be an optimal dose of 5 μg beyond which there is no increase in bone volume.

Original languageEnglish (US)
Pages (from-to)1149-1155
Number of pages7
JournalLaryngoscope
Volume123
Issue number5
DOIs
StatePublished - May 2013

Keywords

  • Biomaterials
  • Level of Evidence: NA
  • craniofacial
  • mandible
  • osteogenesis
  • tissue engineering

ASJC Scopus subject areas

  • Otorhinolaryngology

Fingerprint Dive into the research topics of 'Bone morphogenetic protein-2-impregnated biomimetic scaffolds successfully induce bone healing in a marginal mandibular defect'. Together they form a unique fingerprint.

  • Cite this

    Deconde, A. S., Sidell, D., Lee, M., Bezouglaia, O., Low, K., Elashoff, D., Grogan, T., Tetradis, S., Aghaloo, T., & St. John, M. (2013). Bone morphogenetic protein-2-impregnated biomimetic scaffolds successfully induce bone healing in a marginal mandibular defect. Laryngoscope, 123(5), 1149-1155. https://doi.org/10.1002/lary.23782