Blotting and band-shifting: techniques for studying protein-protein interactions

Daniel Carr, John D. Scott

Research output: Contribution to journalArticle

94 Scopus citations

Abstract

The type II cAMP-dependent protein kinase (PKA) is localized in certain cellular compartments through association with specific A-kinase anchoring proteins (AKAPs). A variety of blotting and electrophoresis techniques have been developed to study the protein-protein interactions that occur between the regulatory (R) subunit of PKA and AKAPs. These methods have also been used for a variety of purposes such as detecting calmodulin-binding proteins, comparing wild-type- and mutant-form binding affinities and estimating the molecular weight of multiprotein complexes.

Original languageEnglish (US)
Pages (from-to)246-249
Number of pages4
JournalTrends in Biochemical Sciences
Volume17
Issue number7
DOIs
Publication statusPublished - 1992

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ASJC Scopus subject areas

  • Biochemistry

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