Blood flow through the embryonic heart outflow tract during cardiac looping in HH13-HH18 chicken embryos

Madeline Midgett, Venkat Keshav Chivukula, Calder Dorn, Samantha Wallace, Sandra Rugonyi

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Blood flow is inherently linked to embryonic cardiac development, as haemodynamic forces exerted by flow stimulate mechanotransduction mechanisms that modulate cardiac growth and remodelling. This study evaluated blood flow in the embryonic heart outflow tract (OFT) during normal development at each stage between HH13 and HH18 in chicken embryos, in order to characterize changes in haemodynamic conditions during critical cardiac looping transformations. Two-dimensional optical coherence tomography was used to simultaneously acquire both structural and Doppler flow images, in order to extract blood flow velocity and structural information and estimate haemodynamic measures. From HH13 to HH18, peak blood flow rate increased by 2.4-fold and stroke volume increased by 2.1-fold. Wall shear rate (WSR) and lumen diameter data suggest that changes in blood flow during HH13-HH18 may induce a shear-mediated vasodilation response in the OFT. Embryo-specific four-dimensional computational fluid dynamics modelling at HH13 and HH18 complemented experimental observations and indicated heterogeneous WSR distributions over the OFT. Characterizing changes in haemodynamics during cardiac looping will help us better understand the way normal blood flow impacts proper cardiac development.

Original languageEnglish (US)
Article number20150652
JournalJournal of the Royal Society Interface
Volume12
Issue number111
DOIs
StatePublished - Oct 6 2015

Fingerprint

Chickens
Blood
Embryonic Structures
Hemodynamics
Shear deformation
Blood Flow Velocity
Optical Coherence Tomography
Hydrodynamics
Vasodilation
Stroke Volume
Optical tomography
Embryonic Development
Flow velocity
Computational fluid dynamics
Flow rate
Growth

Keywords

  • Cardiovascular development
  • Chick embryo
  • Haemodynamics
  • Optical coherence tomography
  • Outflow tract
  • Subject-specific haemodynamic modelling

ASJC Scopus subject areas

  • Biophysics
  • Biotechnology
  • Bioengineering
  • Biomedical Engineering
  • Biomaterials
  • Biochemistry

Cite this

Blood flow through the embryonic heart outflow tract during cardiac looping in HH13-HH18 chicken embryos. / Midgett, Madeline; Chivukula, Venkat Keshav; Dorn, Calder; Wallace, Samantha; Rugonyi, Sandra.

In: Journal of the Royal Society Interface, Vol. 12, No. 111, 20150652, 06.10.2015.

Research output: Contribution to journalArticle

Midgett, Madeline ; Chivukula, Venkat Keshav ; Dorn, Calder ; Wallace, Samantha ; Rugonyi, Sandra. / Blood flow through the embryonic heart outflow tract during cardiac looping in HH13-HH18 chicken embryos. In: Journal of the Royal Society Interface. 2015 ; Vol. 12, No. 111.
@article{2c779d3ee7024886aaf1889225e12a58,
title = "Blood flow through the embryonic heart outflow tract during cardiac looping in HH13-HH18 chicken embryos",
abstract = "Blood flow is inherently linked to embryonic cardiac development, as haemodynamic forces exerted by flow stimulate mechanotransduction mechanisms that modulate cardiac growth and remodelling. This study evaluated blood flow in the embryonic heart outflow tract (OFT) during normal development at each stage between HH13 and HH18 in chicken embryos, in order to characterize changes in haemodynamic conditions during critical cardiac looping transformations. Two-dimensional optical coherence tomography was used to simultaneously acquire both structural and Doppler flow images, in order to extract blood flow velocity and structural information and estimate haemodynamic measures. From HH13 to HH18, peak blood flow rate increased by 2.4-fold and stroke volume increased by 2.1-fold. Wall shear rate (WSR) and lumen diameter data suggest that changes in blood flow during HH13-HH18 may induce a shear-mediated vasodilation response in the OFT. Embryo-specific four-dimensional computational fluid dynamics modelling at HH13 and HH18 complemented experimental observations and indicated heterogeneous WSR distributions over the OFT. Characterizing changes in haemodynamics during cardiac looping will help us better understand the way normal blood flow impacts proper cardiac development.",
keywords = "Cardiovascular development, Chick embryo, Haemodynamics, Optical coherence tomography, Outflow tract, Subject-specific haemodynamic modelling",
author = "Madeline Midgett and Chivukula, {Venkat Keshav} and Calder Dorn and Samantha Wallace and Sandra Rugonyi",
year = "2015",
month = "10",
day = "6",
doi = "10.1098/rsif.2015.0652",
language = "English (US)",
volume = "12",
journal = "Journal of the Royal Society Interface",
issn = "1742-5689",
publisher = "Royal Society of London",
number = "111",

}

TY - JOUR

T1 - Blood flow through the embryonic heart outflow tract during cardiac looping in HH13-HH18 chicken embryos

AU - Midgett, Madeline

AU - Chivukula, Venkat Keshav

AU - Dorn, Calder

AU - Wallace, Samantha

AU - Rugonyi, Sandra

PY - 2015/10/6

Y1 - 2015/10/6

N2 - Blood flow is inherently linked to embryonic cardiac development, as haemodynamic forces exerted by flow stimulate mechanotransduction mechanisms that modulate cardiac growth and remodelling. This study evaluated blood flow in the embryonic heart outflow tract (OFT) during normal development at each stage between HH13 and HH18 in chicken embryos, in order to characterize changes in haemodynamic conditions during critical cardiac looping transformations. Two-dimensional optical coherence tomography was used to simultaneously acquire both structural and Doppler flow images, in order to extract blood flow velocity and structural information and estimate haemodynamic measures. From HH13 to HH18, peak blood flow rate increased by 2.4-fold and stroke volume increased by 2.1-fold. Wall shear rate (WSR) and lumen diameter data suggest that changes in blood flow during HH13-HH18 may induce a shear-mediated vasodilation response in the OFT. Embryo-specific four-dimensional computational fluid dynamics modelling at HH13 and HH18 complemented experimental observations and indicated heterogeneous WSR distributions over the OFT. Characterizing changes in haemodynamics during cardiac looping will help us better understand the way normal blood flow impacts proper cardiac development.

AB - Blood flow is inherently linked to embryonic cardiac development, as haemodynamic forces exerted by flow stimulate mechanotransduction mechanisms that modulate cardiac growth and remodelling. This study evaluated blood flow in the embryonic heart outflow tract (OFT) during normal development at each stage between HH13 and HH18 in chicken embryos, in order to characterize changes in haemodynamic conditions during critical cardiac looping transformations. Two-dimensional optical coherence tomography was used to simultaneously acquire both structural and Doppler flow images, in order to extract blood flow velocity and structural information and estimate haemodynamic measures. From HH13 to HH18, peak blood flow rate increased by 2.4-fold and stroke volume increased by 2.1-fold. Wall shear rate (WSR) and lumen diameter data suggest that changes in blood flow during HH13-HH18 may induce a shear-mediated vasodilation response in the OFT. Embryo-specific four-dimensional computational fluid dynamics modelling at HH13 and HH18 complemented experimental observations and indicated heterogeneous WSR distributions over the OFT. Characterizing changes in haemodynamics during cardiac looping will help us better understand the way normal blood flow impacts proper cardiac development.

KW - Cardiovascular development

KW - Chick embryo

KW - Haemodynamics

KW - Optical coherence tomography

KW - Outflow tract

KW - Subject-specific haemodynamic modelling

UR - http://www.scopus.com/inward/record.url?scp=84945944351&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84945944351&partnerID=8YFLogxK

U2 - 10.1098/rsif.2015.0652

DO - 10.1098/rsif.2015.0652

M3 - Article

C2 - 26468069

AN - SCOPUS:84945944351

VL - 12

JO - Journal of the Royal Society Interface

JF - Journal of the Royal Society Interface

SN - 1742-5689

IS - 111

M1 - 20150652

ER -