Block of glutamate transporters potentiates postsynaptic excitation

Gang Tong, Craig Jahr

Research output: Contribution to journalArticle

284 Citations (Scopus)

Abstract

We have studied the effects of blockers of glutamate transporters on excitatory synaptic transmission to determine whether transporters increase the clearance rate of transmitter from the synaptic cleft on the millisecond time scale. The transporter blockers Li+ and THA increased the amplitude, but not the decay time, of spontaneous miniature AMPA receptor EPSCs recorded at 34°C but not 24°C. Evoked AMPA receptor EPSCs were similarly affected by THA. The rapidly dissociating AMPA receptor competitive antagonist PDA inhibited evoked AMPA receptor EPSCs less in the presence of THA at both temperatures, implying that transporter blockade slows clearance. We suggest that transporters speed glutamate clearance mainly by binding glutamate and that AMPA receptors are not saturated by synaptically released glutamate at 34°C.

Original languageEnglish (US)
Pages (from-to)1195-1203
Number of pages9
JournalNeuron
Volume13
Issue number5
DOIs
StatePublished - 1994

Fingerprint

Amino Acid Transport System X-AG
AMPA Receptors
Tacrine
Glutamate Receptors
Synaptic Transmission
Glutamic Acid
Temperature

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Block of glutamate transporters potentiates postsynaptic excitation. / Tong, Gang; Jahr, Craig.

In: Neuron, Vol. 13, No. 5, 1994, p. 1195-1203.

Research output: Contribution to journalArticle

Tong, Gang ; Jahr, Craig. / Block of glutamate transporters potentiates postsynaptic excitation. In: Neuron. 1994 ; Vol. 13, No. 5. pp. 1195-1203.
@article{2800079bd1c547cdbe4d3de6b2fd8fe7,
title = "Block of glutamate transporters potentiates postsynaptic excitation",
abstract = "We have studied the effects of blockers of glutamate transporters on excitatory synaptic transmission to determine whether transporters increase the clearance rate of transmitter from the synaptic cleft on the millisecond time scale. The transporter blockers Li+ and THA increased the amplitude, but not the decay time, of spontaneous miniature AMPA receptor EPSCs recorded at 34°C but not 24°C. Evoked AMPA receptor EPSCs were similarly affected by THA. The rapidly dissociating AMPA receptor competitive antagonist PDA inhibited evoked AMPA receptor EPSCs less in the presence of THA at both temperatures, implying that transporter blockade slows clearance. We suggest that transporters speed glutamate clearance mainly by binding glutamate and that AMPA receptors are not saturated by synaptically released glutamate at 34°C.",
author = "Gang Tong and Craig Jahr",
year = "1994",
doi = "10.1016/0896-6273(94)90057-4",
language = "English (US)",
volume = "13",
pages = "1195--1203",
journal = "Neuron",
issn = "0896-6273",
publisher = "Cell Press",
number = "5",

}

TY - JOUR

T1 - Block of glutamate transporters potentiates postsynaptic excitation

AU - Tong, Gang

AU - Jahr, Craig

PY - 1994

Y1 - 1994

N2 - We have studied the effects of blockers of glutamate transporters on excitatory synaptic transmission to determine whether transporters increase the clearance rate of transmitter from the synaptic cleft on the millisecond time scale. The transporter blockers Li+ and THA increased the amplitude, but not the decay time, of spontaneous miniature AMPA receptor EPSCs recorded at 34°C but not 24°C. Evoked AMPA receptor EPSCs were similarly affected by THA. The rapidly dissociating AMPA receptor competitive antagonist PDA inhibited evoked AMPA receptor EPSCs less in the presence of THA at both temperatures, implying that transporter blockade slows clearance. We suggest that transporters speed glutamate clearance mainly by binding glutamate and that AMPA receptors are not saturated by synaptically released glutamate at 34°C.

AB - We have studied the effects of blockers of glutamate transporters on excitatory synaptic transmission to determine whether transporters increase the clearance rate of transmitter from the synaptic cleft on the millisecond time scale. The transporter blockers Li+ and THA increased the amplitude, but not the decay time, of spontaneous miniature AMPA receptor EPSCs recorded at 34°C but not 24°C. Evoked AMPA receptor EPSCs were similarly affected by THA. The rapidly dissociating AMPA receptor competitive antagonist PDA inhibited evoked AMPA receptor EPSCs less in the presence of THA at both temperatures, implying that transporter blockade slows clearance. We suggest that transporters speed glutamate clearance mainly by binding glutamate and that AMPA receptors are not saturated by synaptically released glutamate at 34°C.

UR - http://www.scopus.com/inward/record.url?scp=0028061339&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028061339&partnerID=8YFLogxK

U2 - 10.1016/0896-6273(94)90057-4

DO - 10.1016/0896-6273(94)90057-4

M3 - Article

VL - 13

SP - 1195

EP - 1203

JO - Neuron

JF - Neuron

SN - 0896-6273

IS - 5

ER -