Abstract
Brief exposure of covalently closed circular duplex PM2 DNA to low concentrations of the clinical bleomycin mixture (Blenoxane) resulted in specific fragmentation of the genome that does not depend on the presence of superhelical turns. The double-strand breaks are in fact produced at several discrete sites on the PM2 genome but frequently occurring near the HpaII restriction endonuclease cleavage site. Initial rates of formation of nicked circular and linear duplex PM2 DNAs are reduced to different extents as the ionic strength of the reaction is increased. Increasing ionic strength is most effective in reducing the initial rate and overall yield of apparent double-strand scissions compared with single-strand scissions in the bleomycin-treated PM2 DNA.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1890-1896 |
| Number of pages | 7 |
| Journal | Biochemistry |
| Volume | 17 |
| Issue number | 10 |
| State | Published - 1978 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Biochemistry
Cite this
Bleomycin-specific fragmentation of double-stranded DNA. / Lloyd, Robert (Stephen); Haidle, Charles W.; Robberson, Donald L.
In: Biochemistry, Vol. 17, No. 10, 1978, p. 1890-1896.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Bleomycin-specific fragmentation of double-stranded DNA
AU - Lloyd, Robert (Stephen)
AU - Haidle, Charles W.
AU - Robberson, Donald L.
PY - 1978
Y1 - 1978
N2 - Brief exposure of covalently closed circular duplex PM2 DNA to low concentrations of the clinical bleomycin mixture (Blenoxane) resulted in specific fragmentation of the genome that does not depend on the presence of superhelical turns. The double-strand breaks are in fact produced at several discrete sites on the PM2 genome but frequently occurring near the HpaII restriction endonuclease cleavage site. Initial rates of formation of nicked circular and linear duplex PM2 DNAs are reduced to different extents as the ionic strength of the reaction is increased. Increasing ionic strength is most effective in reducing the initial rate and overall yield of apparent double-strand scissions compared with single-strand scissions in the bleomycin-treated PM2 DNA.
AB - Brief exposure of covalently closed circular duplex PM2 DNA to low concentrations of the clinical bleomycin mixture (Blenoxane) resulted in specific fragmentation of the genome that does not depend on the presence of superhelical turns. The double-strand breaks are in fact produced at several discrete sites on the PM2 genome but frequently occurring near the HpaII restriction endonuclease cleavage site. Initial rates of formation of nicked circular and linear duplex PM2 DNAs are reduced to different extents as the ionic strength of the reaction is increased. Increasing ionic strength is most effective in reducing the initial rate and overall yield of apparent double-strand scissions compared with single-strand scissions in the bleomycin-treated PM2 DNA.
UR - http://www.scopus.com/inward/record.url?scp=0018194944&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0018194944&partnerID=8YFLogxK
M3 - Article
C2 - 77675
AN - SCOPUS:0018194944
VL - 17
SP - 1890
EP - 1896
JO - Biochemistry
JF - Biochemistry
SN - 0006-2960
IS - 10
ER -