The amount of breakage induced by bleomycin in closed-circular DNA from bacteriophage PM2 was investigated under various reaction conditions. Analysis of the extent of breakage, by alkaline band centrifugation and agarose gel electrophoresis, yielded quite different results from those previously reported for the breakage of closed-circular SV40 DNA by bleomycin. Compared to these earlier results, we found (a) the reaction required the addition of 2-mercaptoethanol; and (b) the breakage reaction was further stimulated by the addition of 2-mercaptoethanol in the absence of EDTA.
|Original language||English (US)|
|Number of pages||7|
|Journal||Biochemical and Biophysical Research Communications|
|State||Published - Mar 21 1977|
ASJC Scopus subject areas
- Molecular Biology
- Cell Biology