Bivariate flout cytometric analysis of murine intestinal epithelial cells for cytokinetic studies

M. G. Pallavicini, C. R. Ng, J. W. Gray

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

The heterogeneous nature of the small intestine and the lack of methods to obtain pure crypt populations has, in the past, limited the application of standard flow cytometric analysis for cytokinetic studies of the proliferating crypts. We describe a flow cytometric technique to discriminate crypt and villus cells in an epithelial cell suspension on the basis of cell length, and to measure the DNA content of the discriminated subpopulations. Our data indicate that bivariate analysis of a mixed epithelial cell suspension can be used to distinguish mature villus cells, G1 crypt cells, and S‐phase crypt cells. In addition, continuous labeling studies suggest that the position of a cell on the cell length axis reflects epithelial cell maturity. We applied this flow cytometric technique to determine the cvtokinetic nature of epithelial cells obtained by sequential digestion of the small intestine.

Original languageEnglish (US)
Pages (from-to)55-62
Number of pages8
JournalCytometry
Volume5
Issue number1
DOIs
StatePublished - Jan 1984

Keywords

  • cell kinetics
  • Flow cytometry
  • intestinal crypt cells

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Biophysics
  • Hematology
  • Endocrinology
  • Cell Biology

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