Biorheology of Platelet Activation in the Bloodstream Distal to Thrombus Formation

Jevgenia Zilberman-Rudenko, Asako Itakura, Jeevan Maddala, Sandra M. Baker-Groberg, Ralf Vetter, Erik Tucker, Andras Gruber, Christoph Gerdes, Owen McCarty

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Thrombus growth at the site of vascular injury is mediated by the sequential events of platelet recruitment, activation and aggregation concomitant with the initiation of the coagulation cascade, resulting in local thrombin generation and fibrin formation. While the biorheology of a localized thrombus formation has been well studied, it is unclear whether local sites of thrombin generation propagate platelet activation within the bloodstream. In order to study the physical biology of platelet activation downstream of sites of thrombus formation, we developed a platform to measure platelet activation and microaggregate formation in the bloodstream. Our results show that thrombi formed on collagen and tissue factor promote activation and aggregation of platelets in the bloodstream in a convection-dependent manner. Pharmacological inhibition of the coagulation factors (F) X, XI or thrombin dramatically reduced the degree of distal platelet activation and microaggregate formation in the bloodstream without affecting the degree of local platelet deposition and aggregation on a surface of immobilized collagen. Herein we describe the development and an example of the utility of a platform to study platelet activation and microaggregate formation in the bloodstream (convection-limited regime) relative to the local site of thrombus formation.

Original languageEnglish (US)
Pages (from-to)1-13
Number of pages13
JournalCellular and Molecular Bioengineering
DOIs
StateAccepted/In press - May 25 2016

Fingerprint

Platelets
Platelet Activation
Activation
Thrombosis
Chemical activation
Platelet Aggregation
Thrombin
Convection
Aggregation
Agglomeration
Collagen
Coagulation
Factor X
Vascular System Injuries
Thromboplastin
Blood Coagulation Factors
Fibrin
Cascade
Biology
Pharmacology

Keywords

  • Biophysics
  • Factor X
  • Factor XI
  • Physical biology
  • Platelets
  • Shear
  • Thrombin

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Modeling and Simulation

Cite this

Biorheology of Platelet Activation in the Bloodstream Distal to Thrombus Formation. / Zilberman-Rudenko, Jevgenia; Itakura, Asako; Maddala, Jeevan; Baker-Groberg, Sandra M.; Vetter, Ralf; Tucker, Erik; Gruber, Andras; Gerdes, Christoph; McCarty, Owen.

In: Cellular and Molecular Bioengineering, 25.05.2016, p. 1-13.

Research output: Contribution to journalArticle

Zilberman-Rudenko, Jevgenia ; Itakura, Asako ; Maddala, Jeevan ; Baker-Groberg, Sandra M. ; Vetter, Ralf ; Tucker, Erik ; Gruber, Andras ; Gerdes, Christoph ; McCarty, Owen. / Biorheology of Platelet Activation in the Bloodstream Distal to Thrombus Formation. In: Cellular and Molecular Bioengineering. 2016 ; pp. 1-13.
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