Biomimetic scaffolds facilitate healing of critical-sized segmental mandibular defects

Matthew K. Lee, Adam S. Deconde, Min Lee, Christopher M. Walthers, Ali R. Sepahdari, David Elashoff, Tristan Grogan, Olga Bezouglaia, Sotirios Tetradis, Maie St. John, Tara Aghaloo

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Objective To investigate the efficacy of biomimetic PLGA scaffolds, alone and in combination with bone morphogenic protein (BMP-2) and adipose-derived stem cells (ASCs), to heal a critical-sized segmental mandibular defect in a rat model. Study design Prospective animal study. Methods ASCs were isolated and cultured from the inguinal fat of Lewis rat pups. Using three-dimensional printing, PLGA scaffolds were fabricated and impregnated with BMP-2 and/or ASCs. Critical-sized 5-mm segmental mandibular defects were created in adult Lewis rats and implanted with (1) blank PLGA scaffolds, (2) PLGA scaffolds with ASCs, (3) PLGA scaffolds with BMP, or (4) PLGA scaffolds with BMP and ASCs. Animals were sacrificed at 12 weeks. Bone regeneration was assessed using microCT, and graded on a semi-quantitative bone formation and bone union scale. Results Twenty-eight rats underwent creation of segmental mandibular defects with implantation of scaffolds. Nine rats suffered complications and were excluded from analysis, leaving 19 animals for inclusion in the study. MicroCT analysis demonstrated no bridging of the segmental bony defect in rats implanted with blank scaffolds (median bone union score = 0). Rats implanted with scaffolds containing BMP-2 (median bone union = 2.0), ASCs (median bone union = 1.5), and combination of BMP and ASCs (median bone union = 1.0) demonstrated healing of critical-sized segmental mandibular defects. Bone regeneration was most robust in the BMP-2 treated scaffolds. Conclusions The current study utilizes a novel animal model to study the efficacy of biomimetic scaffolds carrying osteogenic factors to induce healing of a critical-sized segmental mandibular defect. Level of evidence N/A, Basic Science Animal Research.

Original languageEnglish (US)
Pages (from-to)1-6
Number of pages6
JournalAmerican Journal of Otolaryngology - Head and Neck Medicine and Surgery
Volume36
Issue number1
DOIs
StatePublished - Jan 1 2015
Externally publishedYes

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Biomimetics
Stem Cells
Bone and Bones
X-Ray Microtomography
Bone Regeneration
Groin
Osteogenesis
polylactic acid-polyglycolic acid copolymer
Animal Models
Fats
Prospective Studies

ASJC Scopus subject areas

  • Otorhinolaryngology
  • Medicine(all)

Cite this

Biomimetic scaffolds facilitate healing of critical-sized segmental mandibular defects. / Lee, Matthew K.; Deconde, Adam S.; Lee, Min; Walthers, Christopher M.; Sepahdari, Ali R.; Elashoff, David; Grogan, Tristan; Bezouglaia, Olga; Tetradis, Sotirios; St. John, Maie; Aghaloo, Tara.

In: American Journal of Otolaryngology - Head and Neck Medicine and Surgery, Vol. 36, No. 1, 01.01.2015, p. 1-6.

Research output: Contribution to journalArticle

Lee, MK, Deconde, AS, Lee, M, Walthers, CM, Sepahdari, AR, Elashoff, D, Grogan, T, Bezouglaia, O, Tetradis, S, St. John, M & Aghaloo, T 2015, 'Biomimetic scaffolds facilitate healing of critical-sized segmental mandibular defects', American Journal of Otolaryngology - Head and Neck Medicine and Surgery, vol. 36, no. 1, pp. 1-6. https://doi.org/10.1016/j.amjoto.2014.06.007
Lee, Matthew K. ; Deconde, Adam S. ; Lee, Min ; Walthers, Christopher M. ; Sepahdari, Ali R. ; Elashoff, David ; Grogan, Tristan ; Bezouglaia, Olga ; Tetradis, Sotirios ; St. John, Maie ; Aghaloo, Tara. / Biomimetic scaffolds facilitate healing of critical-sized segmental mandibular defects. In: American Journal of Otolaryngology - Head and Neck Medicine and Surgery. 2015 ; Vol. 36, No. 1. pp. 1-6.
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abstract = "Objective To investigate the efficacy of biomimetic PLGA scaffolds, alone and in combination with bone morphogenic protein (BMP-2) and adipose-derived stem cells (ASCs), to heal a critical-sized segmental mandibular defect in a rat model. Study design Prospective animal study. Methods ASCs were isolated and cultured from the inguinal fat of Lewis rat pups. Using three-dimensional printing, PLGA scaffolds were fabricated and impregnated with BMP-2 and/or ASCs. Critical-sized 5-mm segmental mandibular defects were created in adult Lewis rats and implanted with (1) blank PLGA scaffolds, (2) PLGA scaffolds with ASCs, (3) PLGA scaffolds with BMP, or (4) PLGA scaffolds with BMP and ASCs. Animals were sacrificed at 12 weeks. Bone regeneration was assessed using microCT, and graded on a semi-quantitative bone formation and bone union scale. Results Twenty-eight rats underwent creation of segmental mandibular defects with implantation of scaffolds. Nine rats suffered complications and were excluded from analysis, leaving 19 animals for inclusion in the study. MicroCT analysis demonstrated no bridging of the segmental bony defect in rats implanted with blank scaffolds (median bone union score = 0). Rats implanted with scaffolds containing BMP-2 (median bone union = 2.0), ASCs (median bone union = 1.5), and combination of BMP and ASCs (median bone union = 1.0) demonstrated healing of critical-sized segmental mandibular defects. Bone regeneration was most robust in the BMP-2 treated scaffolds. Conclusions The current study utilizes a novel animal model to study the efficacy of biomimetic scaffolds carrying osteogenic factors to induce healing of a critical-sized segmental mandibular defect. Level of evidence N/A, Basic Science Animal Research.",
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AU - Elashoff, David

AU - Grogan, Tristan

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AU - Tetradis, Sotirios

AU - St. John, Maie

AU - Aghaloo, Tara

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N2 - Objective To investigate the efficacy of biomimetic PLGA scaffolds, alone and in combination with bone morphogenic protein (BMP-2) and adipose-derived stem cells (ASCs), to heal a critical-sized segmental mandibular defect in a rat model. Study design Prospective animal study. Methods ASCs were isolated and cultured from the inguinal fat of Lewis rat pups. Using three-dimensional printing, PLGA scaffolds were fabricated and impregnated with BMP-2 and/or ASCs. Critical-sized 5-mm segmental mandibular defects were created in adult Lewis rats and implanted with (1) blank PLGA scaffolds, (2) PLGA scaffolds with ASCs, (3) PLGA scaffolds with BMP, or (4) PLGA scaffolds with BMP and ASCs. Animals were sacrificed at 12 weeks. Bone regeneration was assessed using microCT, and graded on a semi-quantitative bone formation and bone union scale. Results Twenty-eight rats underwent creation of segmental mandibular defects with implantation of scaffolds. Nine rats suffered complications and were excluded from analysis, leaving 19 animals for inclusion in the study. MicroCT analysis demonstrated no bridging of the segmental bony defect in rats implanted with blank scaffolds (median bone union score = 0). Rats implanted with scaffolds containing BMP-2 (median bone union = 2.0), ASCs (median bone union = 1.5), and combination of BMP and ASCs (median bone union = 1.0) demonstrated healing of critical-sized segmental mandibular defects. Bone regeneration was most robust in the BMP-2 treated scaffolds. Conclusions The current study utilizes a novel animal model to study the efficacy of biomimetic scaffolds carrying osteogenic factors to induce healing of a critical-sized segmental mandibular defect. Level of evidence N/A, Basic Science Animal Research.

AB - Objective To investigate the efficacy of biomimetic PLGA scaffolds, alone and in combination with bone morphogenic protein (BMP-2) and adipose-derived stem cells (ASCs), to heal a critical-sized segmental mandibular defect in a rat model. Study design Prospective animal study. Methods ASCs were isolated and cultured from the inguinal fat of Lewis rat pups. Using three-dimensional printing, PLGA scaffolds were fabricated and impregnated with BMP-2 and/or ASCs. Critical-sized 5-mm segmental mandibular defects were created in adult Lewis rats and implanted with (1) blank PLGA scaffolds, (2) PLGA scaffolds with ASCs, (3) PLGA scaffolds with BMP, or (4) PLGA scaffolds with BMP and ASCs. Animals were sacrificed at 12 weeks. Bone regeneration was assessed using microCT, and graded on a semi-quantitative bone formation and bone union scale. Results Twenty-eight rats underwent creation of segmental mandibular defects with implantation of scaffolds. Nine rats suffered complications and were excluded from analysis, leaving 19 animals for inclusion in the study. MicroCT analysis demonstrated no bridging of the segmental bony defect in rats implanted with blank scaffolds (median bone union score = 0). Rats implanted with scaffolds containing BMP-2 (median bone union = 2.0), ASCs (median bone union = 1.5), and combination of BMP and ASCs (median bone union = 1.0) demonstrated healing of critical-sized segmental mandibular defects. Bone regeneration was most robust in the BMP-2 treated scaffolds. Conclusions The current study utilizes a novel animal model to study the efficacy of biomimetic scaffolds carrying osteogenic factors to induce healing of a critical-sized segmental mandibular defect. Level of evidence N/A, Basic Science Animal Research.

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