Biodiversity and emerging biogeography of the neutrophilic iron-oxidizing Zetaproteobacteria

Sean M. McAllister, Richard E. Davis, Joyce M. McBeth, Bradley Tebo, David Emerson, Craig L. Moyer

Research output: Contribution to journalArticle

68 Citations (Scopus)

Abstract

Members of the neutrophilic iron-oxidizing candidate class Zetaproteobacteria have predominantly been found at sites of microbially mediated iron oxidation in marine environments around the Pacific Ocean. Eighty-four full-length (>1,400-bp) and 48 partial-length Zetaproteobacteria small-subunit (SSU) rRNA gene sequences from five novel clone libraries, one novel Zetaproteobacteria isolate, and the GenBank database were analyzed to assess the biodiversity of this burgeoning class of the Proteobacteria and to investigate its biogeography between three major sampling regions in the Pacific Ocean: Loihi Seamount, the Southern Mariana Trough, and the Tonga Arc. Sequences were grouped into operational taxonomic units (OTUs) on the basis of a 97% minimum similarity. Of the 28 OTUs detected, 13 were found to be endemic to one of the three main sampling regions and 2 were ubiquitous throughout the Pacific Ocean. Additionally, two deeply rooted OTUs that potentially dominate communities of iron oxidizers originating in the deep subsurface were identified. Spatial autocorrelation analysis and analysis of molecular variance (AMOVA) showed that geographic distance played a significant role in the distribution of Zetaproteobacteria biodiversity, whereas environmental parameters, such as temperature, pH, or total Fe concentration, did not have a significant effect. These results, detected using the coarse resolution of the SSU rRNA gene, indicate that the Zetaproteobacteria have a strong biogeographic signal.

Original languageEnglish (US)
Pages (from-to)5445-5457
Number of pages13
JournalApplied and Environmental Microbiology
Volume77
Issue number15
DOIs
StatePublished - Aug 2011

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Pacific Ocean
Biodiversity
biogeography
Spatial Analysis
Iron
biodiversity
iron
rRNA Genes
Tonga
ocean
Proteobacteria
gene
Nucleic Acid Databases
sampling
seamount
autocorrelation
ribosomal RNA
clone
marine environment
Analysis of Variance

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Food Science
  • Biotechnology
  • Ecology

Cite this

Biodiversity and emerging biogeography of the neutrophilic iron-oxidizing Zetaproteobacteria. / McAllister, Sean M.; Davis, Richard E.; McBeth, Joyce M.; Tebo, Bradley; Emerson, David; Moyer, Craig L.

In: Applied and Environmental Microbiology, Vol. 77, No. 15, 08.2011, p. 5445-5457.

Research output: Contribution to journalArticle

McAllister, Sean M. ; Davis, Richard E. ; McBeth, Joyce M. ; Tebo, Bradley ; Emerson, David ; Moyer, Craig L. / Biodiversity and emerging biogeography of the neutrophilic iron-oxidizing Zetaproteobacteria. In: Applied and Environmental Microbiology. 2011 ; Vol. 77, No. 15. pp. 5445-5457.
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