Biocompatibility of a xenogenic elastin-based biomaterial in a murine implantation model: The role of aluminum chloride pretreatment

Monica Hinds, David W. Courtman, Teresa Goodell, Mason Kwong, Halina Brant-Zawadzki, Allen Burke, Bernard A. Fox, Kenton Gregory

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

We have investigated the long-term effect of aluminum chloride (AlCl 3) treatment on the calcification and inflammatory reaction of a porcine elastin-derived biomaterial (PEB) in a novel subdermal adult mouse model. Untreated PEB disks and PEB treated with AlCl3 were implanted subdermally in BALB/c mice for 30, 60, and 180 days. The calcification of the elastin disks was examined with histological analysis and atomic absorption analysis of calcium content. The inflammatory reaction was evaluated both with histological analysis of explants and by an enzyme-linked immunosorbent assay of the serum in each mouse to determine the production of antielastin antibodies. Robust calcification was evident in all untreated PEBs with calcium levels of 107.1 ± 11.8, 151.4 ± 14.4, and 227.2 ± 23.8 μg/mg for 30, 60, and 180 days, respectively. AlCl3, treatment only temporarily prevented the calcification of the elastin disks for 30 days. By 60 and 180 days, the AlCl3,-treated materials had significant calcification with 88.7 ± 17.4 and 105.3 ± 27.0 μg/mg calcium, respectively. The inflammatory reaction was moderate for both types of implants. The AlCl3-treated implants displayed significantly more macrophage and lymphocyte infiltration at 180 days after implantation, and a trend to higher humoral responses at 30 and 60 days when compared with untreated PEBs. We conclude that PEBs extensively calcify in the adult mice model. AlCl 3, treatment of elastin enhances the long-term immunological response to xenogenic elastin implants and merely delays the onset of calcification.

Original languageEnglish (US)
Pages (from-to)55-64
Number of pages10
JournalJournal of Biomedical Materials Research - Part A
Volume69
Issue number1
StatePublished - Apr 1 2004

Fingerprint

Elastin
Aluminum chloride
Biocompatible Materials
Biocompatibility
Biomaterials
Calcium
Calcification (biochemistry)
Immunosorbents
Lymphocytes
Macrophages
aluminum chloride
Infiltration
Antibodies
Assays
Enzymes

Keywords

  • Biomaterial
  • Calcification
  • Elastin
  • Inflammation
  • Subdermal

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biomaterials

Cite this

Biocompatibility of a xenogenic elastin-based biomaterial in a murine implantation model : The role of aluminum chloride pretreatment. / Hinds, Monica; Courtman, David W.; Goodell, Teresa; Kwong, Mason; Brant-Zawadzki, Halina; Burke, Allen; Fox, Bernard A.; Gregory, Kenton.

In: Journal of Biomedical Materials Research - Part A, Vol. 69, No. 1, 01.04.2004, p. 55-64.

Research output: Contribution to journalArticle

Hinds, Monica ; Courtman, David W. ; Goodell, Teresa ; Kwong, Mason ; Brant-Zawadzki, Halina ; Burke, Allen ; Fox, Bernard A. ; Gregory, Kenton. / Biocompatibility of a xenogenic elastin-based biomaterial in a murine implantation model : The role of aluminum chloride pretreatment. In: Journal of Biomedical Materials Research - Part A. 2004 ; Vol. 69, No. 1. pp. 55-64.
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