Biochemistry and molecular genetics of Leishmania glucose transporters

C. K. Langford, R. J S Burchmore, Scott Landfear, W. Wagner, S. M. Landfear

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Glucose is utilized as a significant source of metabolic energy by Leishmania parasites. This sugar is accumulated by the parasite via a specific carrier-mediated transport system located in the parasite membrane. Parasites may also contain another transporter that shuttles glucose between the cytoplasm and the glycosome, a membrane-bound organelle where the early steps of glycolysis occur. The transport systems of both the insect stage promastigotes and the intracellular amastigotes have been characterized and shown to have kinetic properties that are consistent with the different physiological environments of the insect gut and the macrophage phagolysosome. Several genes have been cloned from Leishmania species which encode proteins with substantial sequence similarity to glucose transporters from mammals and lower eukaryotes. Two of these genes are expressed preferentially in the promastigote stage of the life cycle, where glucose is more readily available and more rapidly transported and metabolized than in the intracellular amastigotes. One of these two developmentally-regulated genes has been functionally expressed in Xenopus oocytes and shown to encode a glucose transporter. A third gene encodes a protein that is also a member of the glucose transporter family on the basis of sequence similarity and proposed secondary structure. However, the significant differences between this protein and the other two suggest that it is likely to transport a different substrate. Functional expression will be required to define the specific biochemical role of each gene within the parasite.

Original languageEnglish (US)
JournalParasitology
Volume108
Issue numberSUPPL.
StatePublished - 1994

Fingerprint

glucose transporters
Leishmania
molecular genetics
Biochemistry
biochemistry
Facilitative Glucose Transport Proteins
Molecular Biology
Parasites
parasites
amastigotes
promastigotes
Genes
genes
glucose
Insects
Microbodies
microbodies
phagosomes
Glucose
Phagosomes

Keywords

  • Gene cloning
  • Glucose transporter
  • Leishmania
  • Membrane proteins

ASJC Scopus subject areas

  • Immunology
  • Parasitology

Cite this

Langford, C. K., Burchmore, R. J. S., Landfear, S., Wagner, W., & Landfear, S. M. (1994). Biochemistry and molecular genetics of Leishmania glucose transporters. Parasitology, 108(SUPPL.).

Biochemistry and molecular genetics of Leishmania glucose transporters. / Langford, C. K.; Burchmore, R. J S; Landfear, Scott; Wagner, W.; Landfear, S. M.

In: Parasitology, Vol. 108, No. SUPPL., 1994.

Research output: Contribution to journalArticle

Langford, CK, Burchmore, RJS, Landfear, S, Wagner, W & Landfear, SM 1994, 'Biochemistry and molecular genetics of Leishmania glucose transporters', Parasitology, vol. 108, no. SUPPL..
Langford CK, Burchmore RJS, Landfear S, Wagner W, Landfear SM. Biochemistry and molecular genetics of Leishmania glucose transporters. Parasitology. 1994;108(SUPPL.).
Langford, C. K. ; Burchmore, R. J S ; Landfear, Scott ; Wagner, W. ; Landfear, S. M. / Biochemistry and molecular genetics of Leishmania glucose transporters. In: Parasitology. 1994 ; Vol. 108, No. SUPPL.
@article{4f21e6d7bf864eea9e5b75e48fdce97f,
title = "Biochemistry and molecular genetics of Leishmania glucose transporters",
abstract = "Glucose is utilized as a significant source of metabolic energy by Leishmania parasites. This sugar is accumulated by the parasite via a specific carrier-mediated transport system located in the parasite membrane. Parasites may also contain another transporter that shuttles glucose between the cytoplasm and the glycosome, a membrane-bound organelle where the early steps of glycolysis occur. The transport systems of both the insect stage promastigotes and the intracellular amastigotes have been characterized and shown to have kinetic properties that are consistent with the different physiological environments of the insect gut and the macrophage phagolysosome. Several genes have been cloned from Leishmania species which encode proteins with substantial sequence similarity to glucose transporters from mammals and lower eukaryotes. Two of these genes are expressed preferentially in the promastigote stage of the life cycle, where glucose is more readily available and more rapidly transported and metabolized than in the intracellular amastigotes. One of these two developmentally-regulated genes has been functionally expressed in Xenopus oocytes and shown to encode a glucose transporter. A third gene encodes a protein that is also a member of the glucose transporter family on the basis of sequence similarity and proposed secondary structure. However, the significant differences between this protein and the other two suggest that it is likely to transport a different substrate. Functional expression will be required to define the specific biochemical role of each gene within the parasite.",
keywords = "Gene cloning, Glucose transporter, Leishmania, Membrane proteins",
author = "Langford, {C. K.} and Burchmore, {R. J S} and Scott Landfear and W. Wagner and Landfear, {S. M.}",
year = "1994",
language = "English (US)",
volume = "108",
journal = "Parasitology",
issn = "0031-1820",
publisher = "Cambridge University Press",
number = "SUPPL.",

}

TY - JOUR

T1 - Biochemistry and molecular genetics of Leishmania glucose transporters

AU - Langford, C. K.

AU - Burchmore, R. J S

AU - Landfear, Scott

AU - Wagner, W.

AU - Landfear, S. M.

PY - 1994

Y1 - 1994

N2 - Glucose is utilized as a significant source of metabolic energy by Leishmania parasites. This sugar is accumulated by the parasite via a specific carrier-mediated transport system located in the parasite membrane. Parasites may also contain another transporter that shuttles glucose between the cytoplasm and the glycosome, a membrane-bound organelle where the early steps of glycolysis occur. The transport systems of both the insect stage promastigotes and the intracellular amastigotes have been characterized and shown to have kinetic properties that are consistent with the different physiological environments of the insect gut and the macrophage phagolysosome. Several genes have been cloned from Leishmania species which encode proteins with substantial sequence similarity to glucose transporters from mammals and lower eukaryotes. Two of these genes are expressed preferentially in the promastigote stage of the life cycle, where glucose is more readily available and more rapidly transported and metabolized than in the intracellular amastigotes. One of these two developmentally-regulated genes has been functionally expressed in Xenopus oocytes and shown to encode a glucose transporter. A third gene encodes a protein that is also a member of the glucose transporter family on the basis of sequence similarity and proposed secondary structure. However, the significant differences between this protein and the other two suggest that it is likely to transport a different substrate. Functional expression will be required to define the specific biochemical role of each gene within the parasite.

AB - Glucose is utilized as a significant source of metabolic energy by Leishmania parasites. This sugar is accumulated by the parasite via a specific carrier-mediated transport system located in the parasite membrane. Parasites may also contain another transporter that shuttles glucose between the cytoplasm and the glycosome, a membrane-bound organelle where the early steps of glycolysis occur. The transport systems of both the insect stage promastigotes and the intracellular amastigotes have been characterized and shown to have kinetic properties that are consistent with the different physiological environments of the insect gut and the macrophage phagolysosome. Several genes have been cloned from Leishmania species which encode proteins with substantial sequence similarity to glucose transporters from mammals and lower eukaryotes. Two of these genes are expressed preferentially in the promastigote stage of the life cycle, where glucose is more readily available and more rapidly transported and metabolized than in the intracellular amastigotes. One of these two developmentally-regulated genes has been functionally expressed in Xenopus oocytes and shown to encode a glucose transporter. A third gene encodes a protein that is also a member of the glucose transporter family on the basis of sequence similarity and proposed secondary structure. However, the significant differences between this protein and the other two suggest that it is likely to transport a different substrate. Functional expression will be required to define the specific biochemical role of each gene within the parasite.

KW - Gene cloning

KW - Glucose transporter

KW - Leishmania

KW - Membrane proteins

UR - http://www.scopus.com/inward/record.url?scp=0027965795&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027965795&partnerID=8YFLogxK

M3 - Article

VL - 108

JO - Parasitology

JF - Parasitology

SN - 0031-1820

IS - SUPPL.

ER -