Biochemical studies on 5'-nucleotidase of Schwann cells in degenerated nerve

S. M. Ross, M. I. Sabri, Peter Spencer

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

This report describes the partial characterization of 5'-nucleotidase (5'-AMPase) in Schwann-cell plasmalemmae (PM) prepared from degenerated cat sciatic nerve. 5'-AMPase was enriched 3.7-fold in the PM fraction over that of the crude homogenate preparation. The plant lectin concanavalin-A (Con-A) reduced Schwann cell PM 5'-AMPase activity in a concentration-dependent manner (30-600 μg/ml). Plasma membrane 5'-AMPase activity was maximally inhibited to 20% of control values by Con-A (400-600 μg/ml), and activity returned to control levels by pretreatment with the hapten sugar α-methyl-D-mannoside (50 mM). Equimolar concentrations of UDP and ADP (100 μM) reduced the rate of hydrolysis of labeled AMP to labeled adenosine in PM to 45% and 35% of controls, respectively. This is the first study to characterize a Schwann-cell PM enzyme and demonstrates that 5'-AMPase may be used as a Schwann-cell PM marker enzyme.

Original languageEnglish (US)
Pages (from-to)324-327
Number of pages4
JournalJournal of Neurochemistry
Volume45
Issue number1
StatePublished - 1985
Externally publishedYes

Fingerprint

5'-Nucleotidase
Schwann Cells
Cells
Concanavalin A
Mannosides
Plant Lectins
Uridine Diphosphate
Haptens
Level control
Sciatic Nerve
Enzymes
Adenosine Monophosphate
Cell membranes
Sugars
Adenosine
Adenosine Diphosphate
Hydrolysis
Cats
Cell Membrane
adenosine monophosphatase

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

Cite this

Biochemical studies on 5'-nucleotidase of Schwann cells in degenerated nerve. / Ross, S. M.; Sabri, M. I.; Spencer, Peter.

In: Journal of Neurochemistry, Vol. 45, No. 1, 1985, p. 324-327.

Research output: Contribution to journalArticle

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