Biochemical and immunological evidence for a calcium pump in chromaffin granules

Steven C. King, Thomas E. Ellenberger, Stanley M. Goldin

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

ATP stimulated the accumulation of 45Ca2+ by chromaffin granule ghosts which contained 5 mM oxalate to trap transported calcium within the lumen. Inasmuch as the ATP-dependent 45Ca2+ transport was resistant to 25 mM ammonium acetate as well as the proton ionophore, carbonylcyanide-m-chlorophenylhydrazone, the chromaffin granule proton translocating ATPase does not provide the energy for this process. Instead, we suggest that chromaffin granules contain a calcium translocating ATPase which catalyzes the 45Ca2+ uptake directly. The observation that chromaffin granules bind to a monoclonal antibody raised against a calcium pump from bovine brain supports this hypothesis.

Original languageEnglish (US)
Pages (from-to)656-663
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume155
Issue number2
DOIs
StatePublished - Sep 15 1988

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ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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