Biochemical and biophysical studies of Bacillus subtilis envelopes under hyperosmotic stress

C. S. López, H. Heras, H. Garda, S. Ruzal, C. Sánchez-Rivas, E. Rivas

Research output: Contribution to journalArticle

49 Scopus citations

Abstract

The behaviour and state of the envelopes from B. subtilis cultures grown in Luria Bertani (LB) medium with and without 1.5 M NaCl are compared. Under hypertonic conditions, the hydrophobicity of the cultures increases. The phospholipid and fatty acid (FA) compositions show important differences: a higher cardiolipin (CL) content [at the expense of phosphatidylglycerol (PG)], and a higher unsaturated and straight chain FA content. The fluidity of the membranes, determined with fluorescent probes, indicates an increase in viscosity of the cytoplasmic membrane. The consequences of these variations in membrane permeability and osmotolerance are discussed. Copyright (C) 2000 Elsevier Science B.V.

Original languageEnglish (US)
Pages (from-to)137-142
Number of pages6
JournalInternational Journal of Food Microbiology
Volume55
Issue number1-3
DOIs
StatePublished - Apr 10 2000

Keywords

  • Bacillus subtilis
  • Fatty acids
  • Membranes
  • Phospholipids
  • Stress

ASJC Scopus subject areas

  • Food Science
  • Microbiology

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