Biochemical and biophysical studies of Bacillus subtilis envelopes under hyperosmotic stress

Claudia Lopez, H. Heras, H. Garda, S. Ruzal, C. Sánchez-Rivas, E. Rivas

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

The behaviour and state of the envelopes from B. subtilis cultures grown in Luria Bertani (LB) medium with and without 1.5 M NaCl are compared. Under hypertonic conditions, the hydrophobicity of the cultures increases. The phospholipid and fatty acid (FA) compositions show important differences: a higher cardiolipin (CL) content [at the expense of phosphatidylglycerol (PG)], and a higher unsaturated and straight chain FA content. The fluidity of the membranes, determined with fluorescent probes, indicates an increase in viscosity of the cytoplasmic membrane. The consequences of these variations in membrane permeability and osmotolerance are discussed. Copyright (C) 2000 Elsevier Science B.V.

Original languageEnglish (US)
Pages (from-to)137-142
Number of pages6
JournalInternational Journal of Food Microbiology
Volume55
Issue number1-3
DOIs
StatePublished - Apr 10 2000
Externally publishedYes

Fingerprint

Bacillus subtilis
Fatty Acids
fatty acid composition
osmotolerance
Phosphatidylglycerols
membrane fluidity
Membrane Fluidity
Cardiolipins
cardiolipins
phosphatidylglycerols
membrane permeability
hydrophobicity
Hydrophobic and Hydrophilic Interactions
Fluorescent Dyes
Viscosity
cell membranes
Permeability
Phospholipids
phospholipids
viscosity

Keywords

  • Bacillus subtilis
  • Fatty acids
  • Membranes
  • Phospholipids
  • Stress

ASJC Scopus subject areas

  • Food Science
  • Microbiology

Cite this

Biochemical and biophysical studies of Bacillus subtilis envelopes under hyperosmotic stress. / Lopez, Claudia; Heras, H.; Garda, H.; Ruzal, S.; Sánchez-Rivas, C.; Rivas, E.

In: International Journal of Food Microbiology, Vol. 55, No. 1-3, 10.04.2000, p. 137-142.

Research output: Contribution to journalArticle

Lopez, Claudia ; Heras, H. ; Garda, H. ; Ruzal, S. ; Sánchez-Rivas, C. ; Rivas, E. / Biochemical and biophysical studies of Bacillus subtilis envelopes under hyperosmotic stress. In: International Journal of Food Microbiology. 2000 ; Vol. 55, No. 1-3. pp. 137-142.
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