TY - JOUR
T1 - Binding of recombinant T cell receptor ligands (RTL) to antigen presenting cells prevents upregulation of CD11b and inhibits T cell activation and transfer of experimental autoimmune encephalomyelitis
AU - Sinha, Sushmita
AU - Miller, Lisa
AU - Subramanian, Sandhya
AU - McCarty, Owen J.T.
AU - Proctor, Thomas
AU - Meza-Romero, Roberto
AU - Huan, Jianya
AU - Burrows, Gregory G.
AU - Vandenbark, Arthur A.
AU - Offner, Halina
N1 - Funding Information:
The authors wish to thank Ms. Eva Niehaus for assistance in preparing the manuscript. Dr. Sinha is a Postdoctoral Fellow of the National Multiple Sclerosis Society and this work was supported in part by the National Multiple Sclerosis Society Postdoctoral Fellowship FG1749-A-1 , grants RG3794-A-4 and RG3794-B-6 ; NIH Grants NS47661 and AI43960; and the Biomedical Laboratory R&D Service, Department of Veterans Affairs .
PY - 2010/8
Y1 - 2010/8
N2 - Recombinant T cell ligands (RTLs) ameliorate experimental autoimmune encephalomyelitis (EAE) in an antigen-specific manner. We evaluated effects of RTL401 (I-As α1β1+PLP-139-151) on splenocytes from SJL/J mice with EAE to study RTL-T cell tolerance-inducing mechanisms. RTLs bound to B, macrophages and DCs, through RTL-MHC-α1β1 moiety. RTL binding reduced CD11b expression on splenic macrophages/DC, and RTL401-conditioned macrophages/DC, not B cells, inhibited T cell activation. Reduced ability of RTL- incubated splenocytes to transfer EAE was likely mediated through macrophages/DC, since B cells were unnecessary for RTL treatment of EAE. These results demonstrate a novel pathway of T cell regulation by RTL-bound APCs.
AB - Recombinant T cell ligands (RTLs) ameliorate experimental autoimmune encephalomyelitis (EAE) in an antigen-specific manner. We evaluated effects of RTL401 (I-As α1β1+PLP-139-151) on splenocytes from SJL/J mice with EAE to study RTL-T cell tolerance-inducing mechanisms. RTLs bound to B, macrophages and DCs, through RTL-MHC-α1β1 moiety. RTL binding reduced CD11b expression on splenic macrophages/DC, and RTL401-conditioned macrophages/DC, not B cells, inhibited T cell activation. Reduced ability of RTL- incubated splenocytes to transfer EAE was likely mediated through macrophages/DC, since B cells were unnecessary for RTL treatment of EAE. These results demonstrate a novel pathway of T cell regulation by RTL-bound APCs.
KW - APCs
KW - EAE
KW - RTLs
KW - Tolerance
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U2 - 10.1016/j.jneuroim.2010.04.013
DO - 10.1016/j.jneuroim.2010.04.013
M3 - Article
C2 - 20546940
AN - SCOPUS:77955653379
SN - 0165-5728
VL - 225
SP - 52
EP - 61
JO - Journal of Neuroimmunology
JF - Journal of Neuroimmunology
IS - 1-2
ER -