Binding of recombinant T cell receptor ligands (RTL) to antigen presenting cells prevents upregulation of CD11b and inhibits T cell activation and transfer of experimental autoimmune encephalomyelitis

Sushmita Sinha, Lisa Miller, Sandhya Subramanian, Owen J.T. McCarty, Thomas Proctor, Roberto Meza-Romero, Jianya Huan, Gregory G. Burrows, Arthur A. Vandenbark, Halina Offner

Research output: Contribution to journalArticle

22 Scopus citations

Abstract

Recombinant T cell ligands (RTLs) ameliorate experimental autoimmune encephalomyelitis (EAE) in an antigen-specific manner. We evaluated effects of RTL401 (I-As α1β1+PLP-139-151) on splenocytes from SJL/J mice with EAE to study RTL-T cell tolerance-inducing mechanisms. RTLs bound to B, macrophages and DCs, through RTL-MHC-α1β1 moiety. RTL binding reduced CD11b expression on splenic macrophages/DC, and RTL401-conditioned macrophages/DC, not B cells, inhibited T cell activation. Reduced ability of RTL- incubated splenocytes to transfer EAE was likely mediated through macrophages/DC, since B cells were unnecessary for RTL treatment of EAE. These results demonstrate a novel pathway of T cell regulation by RTL-bound APCs.

Original languageEnglish (US)
Pages (from-to)52-61
Number of pages10
JournalJournal of Neuroimmunology
Volume225
Issue number1-2
DOIs
StatePublished - Aug 1 2010

Keywords

  • APCs
  • EAE
  • RTLs
  • Tolerance

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Neurology
  • Clinical Neurology

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