Binding of human cytomegalovirus US2 to major histocompatibility complex class I and II proteins is not sufficient for their degradation

Mathieu S. Chevalier, Gwynn M. Daniels, David Johnson

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Human cytomegalovirus (HCMV) glycoprotein US2 causes degradation of major histocompatibility complex (MHC) class I heavy-chain (HC), class II DR-α and DM-α proteins, and HFE, a nonclassical MHC protein. In US2-expressing cells, MHC proteins present in the endoplasmic reticulum (ER) are degraded by cytosolic proteasomes. It appears that US2 binding triggers a normal cellular pathway by which misfolded or aberrant proteins are translocated from the ER to cytoplasmic proteasomes. To better understand how US2 binds MHC proteins and causes their degradation, we constructed a panel of US2 mutants. Mutants truncated from the N terminus as far as residue 40 or from the C terminus to amino acid 140 could bind to class I and class II proteins. Nevertheless, mutants lacking just the cytosolic tail (residues 187 to 199) were unable to cause degradation of both class I and II proteins. Chimeric proteins were constructed in which US2 sequences were replaced with homologous sequences from US3, an HCMV glycoprotein that can also bind to class I and II proteins. One of these US2/US3 chimeras bound to class II but not to class I, and a second bound class I HC better than wild-type US2. Therefore, US2 residues involved in the binding to MHC class I differ subtly from those involved in binding to class II proteins. Moreover, our results demonstrate that the binding of US2 to class I and II proteins is not sufficient to cause degradation of MHC proteins. The cytosolic tail of US2 and certain US2 lumenal sequences, which are not involved in binding to MHC proteins, are required for degradation. Our results are consistent with the hypothesis that US2 couples MHC proteins to components of the ER degradation pathway, enormously increasing the rate of degradation of MHC proteins.

Original languageEnglish (US)
Pages (from-to)8265-8275
Number of pages11
JournalJournal of Virology
Volume76
Issue number16
DOIs
StatePublished - 2002

Fingerprint

Human herpesvirus 5
major histocompatibility complex
Major Histocompatibility Complex
Cytomegalovirus
degradation
Proteins
proteins
Endoplasmic Reticulum
endoplasmic reticulum
Proteasome Endopeptidase Complex
proteasome endopeptidase complex
Tail
mutants
Glycoproteins
glycoproteins
tail
recombinant fusion proteins
chimerism
Sequence Homology
sequence homology

ASJC Scopus subject areas

  • Immunology

Cite this

Binding of human cytomegalovirus US2 to major histocompatibility complex class I and II proteins is not sufficient for their degradation. / Chevalier, Mathieu S.; Daniels, Gwynn M.; Johnson, David.

In: Journal of Virology, Vol. 76, No. 16, 2002, p. 8265-8275.

Research output: Contribution to journalArticle

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