Binding of 3H-naloxone in the mouse brain

Effect of ions and tolerance development

Robert Hitzemann, Barbara A. Hitzemann, Horace H. Loh

Research output: Contribution to journalArticle

90 Citations (Scopus)

Abstract

The binding of 3H-naloxone (spec. act. 5.2 Ci/mmol) in a crude mitochondrial fraction of the whole mouse brain was examined. Binding was reversed by the narcotic agonists levorphanol, morphine and 1-methadone but not by dextrorphan. Levorphanol sensitive (specific) 3H-naloxone binding was blocked by Na+, Li+, Ca++, Mg++ and Mn++ but not by K+. When the crude mitochondrial fraction was separated on a discontinuous sucrose gradient, the highest activity of specific binding was found in the nerve ending particle fraction. Animals made physically dependent by 3 day morphine pellet implantation did not show an increased binding affinity for 3H-nalovxone. The implantation of a 10 mg naloxone pellet increased the apparent total number of binding sites on the second and third day of implantation.

Original languageEnglish (US)
Pages (from-to)2393-2404
Number of pages12
JournalLife Sciences
Volume14
Issue number12
DOIs
StatePublished - Jun 16 1974
Externally publishedYes

Fingerprint

Levorphanol
Naloxone
Brain
Ions
Morphine
Dextrorphan
Nerve Endings
Methadone
Narcotics
Sucrose
Animals
Binding Sites

ASJC Scopus subject areas

  • Pharmacology

Cite this

Binding of 3H-naloxone in the mouse brain : Effect of ions and tolerance development. / Hitzemann, Robert; Hitzemann, Barbara A.; Loh, Horace H.

In: Life Sciences, Vol. 14, No. 12, 16.06.1974, p. 2393-2404.

Research output: Contribution to journalArticle

Hitzemann, Robert ; Hitzemann, Barbara A. ; Loh, Horace H. / Binding of 3H-naloxone in the mouse brain : Effect of ions and tolerance development. In: Life Sciences. 1974 ; Vol. 14, No. 12. pp. 2393-2404.
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