Binding forces of hepatic microsomal and plasma membrane proteins in normal and pancreatitic rats: An AFM force spectroscopic study

Lori A. Slezak, Anthony S. Quinn, Kumudesh C. Sritharan, Jin Ping Wang, Gudrun Aspelund, Douglas J. Taatjes, Dana Andersen

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The docking and fusion of membrane-bound vesicles at the cell plasma membrane are brought about by several participating vesicle membrane, plasma membrane, and soluble cytosolic proteins. An understanding of the interactions between these participating proteins will provide an estimate of the potency and efficacy of secretory vesicle docking and fusion at the plasma membrane in cells of a given tissue. Earlier studies suggest that in chronic pancreatitis, glucose intolerance may be associated with impaired exocytosis/endocytosis of hepatic insulin receptor and glucose transporter proteins. In this study, the binding force profiles between microsome membrane proteins and plasma membrane proteins in liver obtained from normal and pancreatitic rats have been examined using atomic force microscopy. The ability of a VAMP-specific antibody to alter binding between microsome- and plasma membrane-associated membrane proteins was examined. In pancreatitic livers, a significant loss in microsome-plasma membrane binding is observed. Furthermore, our study shows that, in contrast to control livers, the microsome-plasma membrane binding in pancreatitic livers is VAMP-independent, which suggests an absence of VAMP participation in membrane-microsome binding. In confirmation with our earlier findings, these studies suggest altered membrane recycling in liver of rats with chronic pancreatitis.

Original languageEnglish (US)
Pages (from-to)363-367
Number of pages5
JournalMicroscopy Research and Technique
Volume44
Issue number5
DOIs
StatePublished - Mar 1 1999
Externally publishedYes

Fingerprint

Cell membranes
membrane proteins
rats
blood proteins
Rats
Blood Proteins
Membrane Proteins
plasma membrane
Cell Membrane
atomic force microscopy
membranes
proteins
Proteins
microsomes
liver
Liver
Microsomes
Membranes
pancreatitis
Chronic Pancreatitis

Keywords

  • Atomic force microscopy
  • Chronic pancreatitis
  • Liver microsome
  • Membrane binding

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Anatomy
  • Instrumentation

Cite this

Binding forces of hepatic microsomal and plasma membrane proteins in normal and pancreatitic rats : An AFM force spectroscopic study. / Slezak, Lori A.; Quinn, Anthony S.; Sritharan, Kumudesh C.; Wang, Jin Ping; Aspelund, Gudrun; Taatjes, Douglas J.; Andersen, Dana.

In: Microscopy Research and Technique, Vol. 44, No. 5, 01.03.1999, p. 363-367.

Research output: Contribution to journalArticle

Slezak, Lori A. ; Quinn, Anthony S. ; Sritharan, Kumudesh C. ; Wang, Jin Ping ; Aspelund, Gudrun ; Taatjes, Douglas J. ; Andersen, Dana. / Binding forces of hepatic microsomal and plasma membrane proteins in normal and pancreatitic rats : An AFM force spectroscopic study. In: Microscopy Research and Technique. 1999 ; Vol. 44, No. 5. pp. 363-367.
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