Bias from removing read duplication in ultra-deep sequencing experiments

Wanding Zhou, Tenghui Chen, Hao Zhao, Agda Karina Eterovic, Funda Meric-Bernstam, Gordon Mills, Ken Chen

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Motivation: Identifying subclonal mutations and their implications requires accurate estimation of mutant allele fractions from possibly duplicated sequencing reads. Removing duplicate reads assumes that polymerase chain reaction amplification from library constructions is the primary source. The alternative-sampling coincidence from DNA fragmentation-has not been systematically investigated. Results: With sufficiently high-sequencing depth, sampling-induced read duplication is non-negligible, and removing duplicate reads can overcorrect read counts, causing systemic biases in variant allele fraction and copy number variation estimations. Minimal overcorrection occurs when duplicate reads are identified accounting for their mate reads, inserts are of a variety of lengths and samples are sequenced in separate batches. We investigate sampling-induced read duplication in deep sequencing data with 500 to 2000 duplicates-removed sequence coverage. We provide a quantitative solution to overcorrection and guidance for effective designs of deep sequencing platforms that facilitate accurate estimation of variant allele fraction and copy number variation.

Original languageEnglish (US)
Pages (from-to)1073-1080
Number of pages8
JournalBioinformatics
Volume30
Issue number8
DOIs
StatePublished - Jan 1 2014
Externally publishedYes

Fingerprint

High-Throughput Nucleotide Sequencing
Duplication
Sequencing
Alleles
Sampling
Experiment
Experiments
Polymerase chain reaction
DNA Fragmentation
Libraries
Polymerase Chain Reaction
Amplification
DNA
Coincidence
Fragmentation
Mutant
Batch
Guidance
Count
Mutation

ASJC Scopus subject areas

  • Statistics and Probability
  • Biochemistry
  • Molecular Biology
  • Computer Science Applications
  • Computational Theory and Mathematics
  • Computational Mathematics

Cite this

Zhou, W., Chen, T., Zhao, H., Eterovic, A. K., Meric-Bernstam, F., Mills, G., & Chen, K. (2014). Bias from removing read duplication in ultra-deep sequencing experiments. Bioinformatics, 30(8), 1073-1080. https://doi.org/10.1093/bioinformatics/btt771

Bias from removing read duplication in ultra-deep sequencing experiments. / Zhou, Wanding; Chen, Tenghui; Zhao, Hao; Eterovic, Agda Karina; Meric-Bernstam, Funda; Mills, Gordon; Chen, Ken.

In: Bioinformatics, Vol. 30, No. 8, 01.01.2014, p. 1073-1080.

Research output: Contribution to journalArticle

Zhou, W, Chen, T, Zhao, H, Eterovic, AK, Meric-Bernstam, F, Mills, G & Chen, K 2014, 'Bias from removing read duplication in ultra-deep sequencing experiments', Bioinformatics, vol. 30, no. 8, pp. 1073-1080. https://doi.org/10.1093/bioinformatics/btt771
Zhou, Wanding ; Chen, Tenghui ; Zhao, Hao ; Eterovic, Agda Karina ; Meric-Bernstam, Funda ; Mills, Gordon ; Chen, Ken. / Bias from removing read duplication in ultra-deep sequencing experiments. In: Bioinformatics. 2014 ; Vol. 30, No. 8. pp. 1073-1080.
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