BH3-only proteins Noxa, Bik, Bmf, and Bid activate Bax and Bak indirectly when studied in yeast model

Gabriela Gérecová, Jana Kopanicová, Petra Jaká, Lucia Běhalová, Barbora Juhásová, Ingrid Bhatia-Kiššová, Michael Forte, Peter Polčic, Marek Mentel

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

BH3-only proteins of the Bcl-2 family regulate programmed cell death in mammals through activation of multidomain proapoptotic proteins Bax and Bak in response to various proapoptotic stimuli by mechanism that remains under dispute. Here, we report that the cell death-promoting activity of BH3-only proteins Bik, Bmf, Noxa, and tBid can only be reconstituted in yeast when both multidomain proapoptotic and antiapoptotic Bcl-2 family proteins are present. Inability of these proteins to induce cell death in the absence of antiapoptotic proteins suggests that all tested BH3-only proteins likely activate Bax and Bak indirectly by inhibiting antiapoptotic proteins.

Original languageEnglish (US)
Pages (from-to)747-754
Number of pages8
JournalFEMS Yeast Research
Volume13
Issue number8
DOIs
StatePublished - Dec 1 2013

    Fingerprint

Keywords

  • Apoptosis
  • BH3-only proteins
  • Bax
  • Bcl-X
  • Yeast

ASJC Scopus subject areas

  • Microbiology
  • Applied Microbiology and Biotechnology

Cite this

Gérecová, G., Kopanicová, J., Jaká, P., Běhalová, L., Juhásová, B., Bhatia-Kiššová, I., Forte, M., Polčic, P., & Mentel, M. (2013). BH3-only proteins Noxa, Bik, Bmf, and Bid activate Bax and Bak indirectly when studied in yeast model. FEMS Yeast Research, 13(8), 747-754. https://doi.org/10.1111/1567-1364.12074