BH3-only protein Bim inhibits activity of antiapoptotic members of Bcl-2 family when expressed in yeast

Barbora Juhásová, Marek Mentel, Ingrid Bhatia-Kiššová, Igor Zeman, Jordan Kolarov, Michael Forte, Peter Polčic

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

Proteins of the Bcl-2 family regulate programmed cell death in mammals by promoting the release of cytochrome c from mitochondria in response to various proapoptotic stimuli. The mechanism by which BH3-only members of the family activate multidomain proapoptotic proteins Bax and Bak to form a pore in mitochondrial membranes remains under dispute. We report that cell death promoting activity of BH3-only protein Bim can be reconstituted in yeast when both Bax and antiapoptotic protein Bcl-XL are present, suggesting that Bim likely activates Bax indirectly by inhibiting antiapoptotic proteins.

Original languageEnglish (US)
Pages (from-to)2709-2713
Number of pages5
JournalFEBS Letters
Volume585
Issue number17
DOIs
StatePublished - Sep 2 2011

Keywords

  • Apoptosis
  • BH3-only proteins
  • Bax
  • Bcl-X
  • Bim
  • Yeast

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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    Juhásová, B., Mentel, M., Bhatia-Kiššová, I., Zeman, I., Kolarov, J., Forte, M., & Polčic, P. (2011). BH3-only protein Bim inhibits activity of antiapoptotic members of Bcl-2 family when expressed in yeast. FEBS Letters, 585(17), 2709-2713. https://doi.org/10.1016/j.febslet.2011.07.027