Abstract
Proteins of the Bcl-2 family regulate programmed cell death in mammals by promoting the release of cytochrome c from mitochondria in response to various proapoptotic stimuli. The mechanism by which BH3-only members of the family activate multidomain proapoptotic proteins Bax and Bak to form a pore in mitochondrial membranes remains under dispute. We report that cell death promoting activity of BH3-only protein Bim can be reconstituted in yeast when both Bax and antiapoptotic protein Bcl-XL are present, suggesting that Bim likely activates Bax indirectly by inhibiting antiapoptotic proteins.
Original language | English (US) |
---|---|
Pages (from-to) | 2709-2713 |
Number of pages | 5 |
Journal | FEBS Letters |
Volume | 585 |
Issue number | 17 |
DOIs | |
State | Published - Sep 2 2011 |
Keywords
- Apoptosis
- BH3-only proteins
- Bax
- Bcl-X
- Bim
- Yeast
ASJC Scopus subject areas
- Biophysics
- Structural Biology
- Biochemistry
- Molecular Biology
- Genetics
- Cell Biology