Beyond the uterine environment: A nonhuman primate model to investigate maternal-fetal and neonatal outcomes following chronic intrauterine infection

Meredith A. Kelleher, Zheng Liu, Xiaojie Wang, Christopher D. Kroenke, Lisa A. Houser, Brandy L. Dozier, Lauren D. Martin, Ken B. Waites, Cindy Mcevoy, Robert L. Schelonka, Peta L. Grigsby

Research output: Research - peer-reviewArticle

Abstract

BackgroundIntrauterine infection is a significant cause of early preterm birth. We have developed a fetal-neonatal model in the rhesus macaque to determine the impact of chronic intrauterine infection with Ureaplasma parvum on early neonatal reflexes and brain development.MethodsTime-mated, pregnant rhesus macaques were randomized to be inoculated with U. parvum (serovar 1; 10 5 c.f.u.) or control media at ∼120 days' gestational age (dGA). Neonates were delivered by elective hysterotomy at 135-147 dGA (term=167d), stabilized, and cared for in our nonhuman primate neonatal intensive care unit. Neonatal reflex behaviors were assessed from birth, and fetal and postnatal brain magnetic resonance imaging (MRI) was performed.ResultsA total of 13 preterm and 5 term macaque infants were included in the study. Ten preterm infants survived to 6 months of age. U. parvum-infected preterm neonates required more intensive respiratory support than did control infants. MRI studies suggested a potential perturbation of brain growth and white matter maturation with exposure to intra-amniotic infection.ConclusionWe have demonstrated the feasibility of longitudinal fetal-neonatal studies in the preterm rhesus macaque after chronic intrauterine infection. Future studies will examine long-term neurobehavioral outcomes, cognitive development, neuropathology, and in vivo brain imaging to determine the safety of antenatal antibiotic treatment for intrauterine infection.

LanguageEnglish (US)
Pages244-252
Number of pages9
JournalPediatric Research
Volume82
Issue number2
DOIs
StatePublished - Aug 1 2017

Fingerprint

Primates
Mothers
Infection
Ureaplasma
Macaca mulatta
Brain
Gestational Age
Reflex
Magnetic Resonance Imaging
Newborn Infant
Hysterotomy
Neonatal Intensive Care Units
Premature Birth
Macaca
Premature Infants
Neuroimaging
Parturition
Anti-Bacterial Agents
Safety
Growth

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health

Cite this

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title = "Beyond the uterine environment: A nonhuman primate model to investigate maternal-fetal and neonatal outcomes following chronic intrauterine infection",
abstract = "BackgroundIntrauterine infection is a significant cause of early preterm birth. We have developed a fetal-neonatal model in the rhesus macaque to determine the impact of chronic intrauterine infection with Ureaplasma parvum on early neonatal reflexes and brain development.MethodsTime-mated, pregnant rhesus macaques were randomized to be inoculated with U. parvum (serovar 1; 10 5 c.f.u.) or control media at ∼120 days' gestational age (dGA). Neonates were delivered by elective hysterotomy at 135-147 dGA (term=167d), stabilized, and cared for in our nonhuman primate neonatal intensive care unit. Neonatal reflex behaviors were assessed from birth, and fetal and postnatal brain magnetic resonance imaging (MRI) was performed.ResultsA total of 13 preterm and 5 term macaque infants were included in the study. Ten preterm infants survived to 6 months of age. U. parvum-infected preterm neonates required more intensive respiratory support than did control infants. MRI studies suggested a potential perturbation of brain growth and white matter maturation with exposure to intra-amniotic infection.ConclusionWe have demonstrated the feasibility of longitudinal fetal-neonatal studies in the preterm rhesus macaque after chronic intrauterine infection. Future studies will examine long-term neurobehavioral outcomes, cognitive development, neuropathology, and in vivo brain imaging to determine the safety of antenatal antibiotic treatment for intrauterine infection.",
author = "Kelleher, {Meredith A.} and Zheng Liu and Xiaojie Wang and Kroenke, {Christopher D.} and Houser, {Lisa A.} and Dozier, {Brandy L.} and Martin, {Lauren D.} and Waites, {Ken B.} and Cindy Mcevoy and Schelonka, {Robert L.} and Grigsby, {Peta L.}",
year = "2017",
month = "8",
doi = "10.1038/pr.2017.57",
volume = "82",
pages = "244--252",
journal = "Pediatric Research",
issn = "0031-3998",
publisher = "Lippincott Williams and Wilkins",
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T1 - Beyond the uterine environment

T2 - Pediatric Research

AU - Kelleher,Meredith A.

AU - Liu,Zheng

AU - Wang,Xiaojie

AU - Kroenke,Christopher D.

AU - Houser,Lisa A.

AU - Dozier,Brandy L.

AU - Martin,Lauren D.

AU - Waites,Ken B.

AU - Mcevoy,Cindy

AU - Schelonka,Robert L.

AU - Grigsby,Peta L.

PY - 2017/8/1

Y1 - 2017/8/1

N2 - BackgroundIntrauterine infection is a significant cause of early preterm birth. We have developed a fetal-neonatal model in the rhesus macaque to determine the impact of chronic intrauterine infection with Ureaplasma parvum on early neonatal reflexes and brain development.MethodsTime-mated, pregnant rhesus macaques were randomized to be inoculated with U. parvum (serovar 1; 10 5 c.f.u.) or control media at ∼120 days' gestational age (dGA). Neonates were delivered by elective hysterotomy at 135-147 dGA (term=167d), stabilized, and cared for in our nonhuman primate neonatal intensive care unit. Neonatal reflex behaviors were assessed from birth, and fetal and postnatal brain magnetic resonance imaging (MRI) was performed.ResultsA total of 13 preterm and 5 term macaque infants were included in the study. Ten preterm infants survived to 6 months of age. U. parvum-infected preterm neonates required more intensive respiratory support than did control infants. MRI studies suggested a potential perturbation of brain growth and white matter maturation with exposure to intra-amniotic infection.ConclusionWe have demonstrated the feasibility of longitudinal fetal-neonatal studies in the preterm rhesus macaque after chronic intrauterine infection. Future studies will examine long-term neurobehavioral outcomes, cognitive development, neuropathology, and in vivo brain imaging to determine the safety of antenatal antibiotic treatment for intrauterine infection.

AB - BackgroundIntrauterine infection is a significant cause of early preterm birth. We have developed a fetal-neonatal model in the rhesus macaque to determine the impact of chronic intrauterine infection with Ureaplasma parvum on early neonatal reflexes and brain development.MethodsTime-mated, pregnant rhesus macaques were randomized to be inoculated with U. parvum (serovar 1; 10 5 c.f.u.) or control media at ∼120 days' gestational age (dGA). Neonates were delivered by elective hysterotomy at 135-147 dGA (term=167d), stabilized, and cared for in our nonhuman primate neonatal intensive care unit. Neonatal reflex behaviors were assessed from birth, and fetal and postnatal brain magnetic resonance imaging (MRI) was performed.ResultsA total of 13 preterm and 5 term macaque infants were included in the study. Ten preterm infants survived to 6 months of age. U. parvum-infected preterm neonates required more intensive respiratory support than did control infants. MRI studies suggested a potential perturbation of brain growth and white matter maturation with exposure to intra-amniotic infection.ConclusionWe have demonstrated the feasibility of longitudinal fetal-neonatal studies in the preterm rhesus macaque after chronic intrauterine infection. Future studies will examine long-term neurobehavioral outcomes, cognitive development, neuropathology, and in vivo brain imaging to determine the safety of antenatal antibiotic treatment for intrauterine infection.

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