Beta adrenergic receptor binding on polymorphonuclear leukocytes in atopic dermatitis

S. P. Galant, S. Underwood, S. Allred, Jon Hanifin

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

It has been postulated that the patient with atopic dermatitis has defective beta adrenergic receptor function. However, a more generalized defect is suggested by the observation that cyclic AMP generation is diminished in these patients following stimulation with both isoproterenol and PGE1. To determine the nature of this abnormality, we measured beta adrenergic receptor binding directly on polymorphonuclear leukocyte membranes using the radiolabeled beta adrenergic antagonist (-) [:3H] dihydroalprenolol (DHA). DHA binding was studied in 6 mild and 9 moderate-to-severe atopic dermatitis patients, and 8 normal controls using a subsaturating concentration of DHA (0.5 nM) to estimate receptor affinity and a saturating concentration of DHA (30 mM) to determine the total number of receptors per cell. No significant differences (p > .05) were found in the total number of receptors per PMN between the control population (805 ± 95) and the mild atopic dermatitis patients (745 ± 91) or the moderate to severe group (621 ± 79). In addition, no significant differences in receptor affinity were found among any of the 3 study groups. These findings suggest that beta receptor binding in atopic dermatitis is normal. Reduced cyclic AMP generation in atopic dermatitis PMN leukocytes would appear to be due to a defect distal to the beta adrenergic receptor itself.

Original languageEnglish (US)
Pages (from-to)330-332
Number of pages3
JournalJournal of Investigative Dermatology
Volume72
Issue number6
StatePublished - 1979
Externally publishedYes

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Dihydroalprenolol
Receptors, Adrenergic, beta
Atopic Dermatitis
Neutrophils
Cyclic AMP
Defects
Adrenergic beta-Antagonists
Alprostadil
Isoproterenol
Leukocytes
Membranes
Population

ASJC Scopus subject areas

  • Dermatology

Cite this

Beta adrenergic receptor binding on polymorphonuclear leukocytes in atopic dermatitis. / Galant, S. P.; Underwood, S.; Allred, S.; Hanifin, Jon.

In: Journal of Investigative Dermatology, Vol. 72, No. 6, 1979, p. 330-332.

Research output: Contribution to journalArticle

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