Best practices for managing large CryoEM facilities

Bart Alewijnse, Alun W. Ashton, Melissa G. Chambers, Songye Chen, Anchi Cheng, Mark Ebrahim, Edward T. Eng, Wim J.H. Hagen, Abraham J. Koster, Claudia Lopez, Natalya Lukoyanova, Joaquin Ortega, Ludovic Renault, Steve Reyntjens, William J. Rice, Giovanna Scapin, Raymond Schrijver, Alistair Siebert, Scott M. Stagg, Valerie Grum-TokarsElizabeth R. Wright, Shenping Wu, Zhiheng Yu, Z. Hong Zhou, Bridget Carragher, Clinton S. Potter

Research output: Contribution to journalReview article

7 Citations (Scopus)

Abstract

This paper provides an overview of the discussion and presentations from the Workshop on the Management of Large CryoEM Facilities held at the New York Structural Biology Center, New York, NY on February 6–7, 2017. A major objective of the workshop was to discuss best practices for managing cryoEM facilities. The discussions were largely focused on supporting single-particle methods for cryoEM and topics included: user access, assessing projects, workflow, sample handling, microscopy, data management and processing, and user training.

Original languageEnglish (US)
Pages (from-to)225-236
Number of pages12
JournalJournal of Structural Biology
Volume199
Issue number3
DOIs
StatePublished - Sep 1 2017

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Practice Guidelines
Education
Workflow
Microscopy

Keywords

  • Automation
  • Cryotem
  • Data management
  • Facility management
  • Single-particle
  • User training

ASJC Scopus subject areas

  • Structural Biology

Cite this

Alewijnse, B., Ashton, A. W., Chambers, M. G., Chen, S., Cheng, A., Ebrahim, M., ... Potter, C. S. (2017). Best practices for managing large CryoEM facilities. Journal of Structural Biology, 199(3), 225-236. https://doi.org/10.1016/j.jsb.2017.07.011

Best practices for managing large CryoEM facilities. / Alewijnse, Bart; Ashton, Alun W.; Chambers, Melissa G.; Chen, Songye; Cheng, Anchi; Ebrahim, Mark; Eng, Edward T.; Hagen, Wim J.H.; Koster, Abraham J.; Lopez, Claudia; Lukoyanova, Natalya; Ortega, Joaquin; Renault, Ludovic; Reyntjens, Steve; Rice, William J.; Scapin, Giovanna; Schrijver, Raymond; Siebert, Alistair; Stagg, Scott M.; Grum-Tokars, Valerie; Wright, Elizabeth R.; Wu, Shenping; Yu, Zhiheng; Zhou, Z. Hong; Carragher, Bridget; Potter, Clinton S.

In: Journal of Structural Biology, Vol. 199, No. 3, 01.09.2017, p. 225-236.

Research output: Contribution to journalReview article

Alewijnse, B, Ashton, AW, Chambers, MG, Chen, S, Cheng, A, Ebrahim, M, Eng, ET, Hagen, WJH, Koster, AJ, Lopez, C, Lukoyanova, N, Ortega, J, Renault, L, Reyntjens, S, Rice, WJ, Scapin, G, Schrijver, R, Siebert, A, Stagg, SM, Grum-Tokars, V, Wright, ER, Wu, S, Yu, Z, Zhou, ZH, Carragher, B & Potter, CS 2017, 'Best practices for managing large CryoEM facilities', Journal of Structural Biology, vol. 199, no. 3, pp. 225-236. https://doi.org/10.1016/j.jsb.2017.07.011
Alewijnse B, Ashton AW, Chambers MG, Chen S, Cheng A, Ebrahim M et al. Best practices for managing large CryoEM facilities. Journal of Structural Biology. 2017 Sep 1;199(3):225-236. https://doi.org/10.1016/j.jsb.2017.07.011
Alewijnse, Bart ; Ashton, Alun W. ; Chambers, Melissa G. ; Chen, Songye ; Cheng, Anchi ; Ebrahim, Mark ; Eng, Edward T. ; Hagen, Wim J.H. ; Koster, Abraham J. ; Lopez, Claudia ; Lukoyanova, Natalya ; Ortega, Joaquin ; Renault, Ludovic ; Reyntjens, Steve ; Rice, William J. ; Scapin, Giovanna ; Schrijver, Raymond ; Siebert, Alistair ; Stagg, Scott M. ; Grum-Tokars, Valerie ; Wright, Elizabeth R. ; Wu, Shenping ; Yu, Zhiheng ; Zhou, Z. Hong ; Carragher, Bridget ; Potter, Clinton S. / Best practices for managing large CryoEM facilities. In: Journal of Structural Biology. 2017 ; Vol. 199, No. 3. pp. 225-236.
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