B‐Cell Abnormalities in AIDS: Stable and Clonally‐Restricted Antibody Response in HIV‐1 Infection

S. MÜLLER, H. WANG, G. J. SILVERMAN, G. BRAMLET, N. HAIGWOOD, H. KÜHLER

Research output: Contribution to journalArticlepeer-review

60 Scopus citations

Abstract

Antibodies in sera of HIV‐1 infected individuals against the HIV‐1 core protein (p24), HIV‐1 envelope gtycoprotein (gp120) and reverse transcriptase (RT) are characterized by a skewed light chain isotype expression. The kappa/lambda ratios of antibodies to p24 and gp120 in infected individuals were found to be unique in each individual, but constant over several years independently from disease progression. The oligoclonal nature of the anti‐HIV‐1 antibodies suggested by skewed kappa/lambda expression was confirmed with isoelectric focusing of affinity purified antibodies to p24 and gp120. Analysis of the utilization of V gene families in purified anti‐p24 and anti‐gp120 antibodies revealed a restricted and biased VH gene family usage. In contrast, the utilization of VK gene families appeared to be random. The finding of stable and restricted antibody responses in infected individuals could be one of the causes for the failure to produce antibodies to HIV‐1 that are effective against escape virus variants.

Original languageEnglish (US)
Pages (from-to)327-334
Number of pages8
JournalScandinavian Journal of Immunology
Volume38
Issue number4
DOIs
StatePublished - Oct 1993
Externally publishedYes

ASJC Scopus subject areas

  • Immunology

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