Batch-invariant nuclear segmentation in whole mount histology sections

Hang Chang, Leandro A. Loss, Paul T. Spellman, Alexander Borowsky, Bahram Parvin

Research output: Chapter in Book/Report/Conference proceedingConference contribution

8 Scopus citations

Abstract

The Cancer Genome Atlas (TCGA) provides a rich repository of whole mount tumor sections that are collected from different laboratories. However, there are a significant amount of technical and biological variations that impede analysis. We have developed a novel approach for nuclear segmentation in histology sections, which addresses the problem of technical and biological variations by incorporating information from manually annotated reference patches with the local color space of the original image. Subsequently, the problem is formulated within a multi-reference graph cut with geodesic constraints. This approach has been validated on manually curated samples and then applied to a dataset of 440 whole mount tissue sections, originating from different laboratories, which are typically 40k-by-40k pixels or larger. Segmentation results, through a zoomable interface, and extracted morphometric data are available at: http://tcga.lbl.gov.

Original languageEnglish (US)
Title of host publication2012 9th IEEE International Symposium on Biomedical Imaging
Subtitle of host publicationFrom Nano to Macro, ISBI 2012 - Proceedings
Pages856-859
Number of pages4
DOIs
StatePublished - Aug 15 2012
Event2012 9th IEEE International Symposium on Biomedical Imaging: From Nano to Macro, ISBI 2012 - Barcelona, Spain
Duration: May 2 2012May 5 2012

Publication series

NameProceedings - International Symposium on Biomedical Imaging
ISSN (Print)1945-7928
ISSN (Electronic)1945-8452

Other

Other2012 9th IEEE International Symposium on Biomedical Imaging: From Nano to Macro, ISBI 2012
CountrySpain
CityBarcelona
Period5/2/125/5/12

Keywords

  • H&E tissue section
  • Nuclear segmentation
  • Nuclear/Background classification

ASJC Scopus subject areas

  • Biomedical Engineering
  • Radiology Nuclear Medicine and imaging

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  • Cite this

    Chang, H., Loss, L. A., Spellman, P. T., Borowsky, A., & Parvin, B. (2012). Batch-invariant nuclear segmentation in whole mount histology sections. In 2012 9th IEEE International Symposium on Biomedical Imaging: From Nano to Macro, ISBI 2012 - Proceedings (pp. 856-859). [6235683] (Proceedings - International Symposium on Biomedical Imaging). https://doi.org/10.1109/ISBI.2012.6235683