Basolateral potassium channel in turtle colon. Evidence for single-file ion flow

K. L. Kirk, D. C. Dawson

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

Treatment of the apical surface of the isolated, ouabain-inhibited turtle colon with the polyene antibiotic amphotericin B permitted the properties of a barium-sensitive potassium conductance in the basolateral membrane to be discerned from the measurements of transepithelial fluxes and electrical currents. Simultaneous measurements of potassium currents and 42K fluxes showed that the movement of potassium was not in accord with simple diffusion. Two other cations, thallium and rubidium, were also permeable and, in addition, exhibited strong interactions with the potassium tracer fluxes. The results indicate that permeant cations exhibit positive coupling, which is consistent with a single-file mechanism of ion translocation through a membrane channel.

Original languageEnglish (US)
Pages (from-to)297-313
Number of pages17
JournalJournal of General Physiology
Volume82
Issue number3
StatePublished - 1983
Externally publishedYes

Fingerprint

Turtles
Potassium Channels
Potassium
Colon
Ions
Cations
Rubidium
Polyenes
Thallium
Amphotericin B
Ouabain
Barium
Ion Channels
Anti-Bacterial Agents
Membranes

ASJC Scopus subject areas

  • Physiology

Cite this

Basolateral potassium channel in turtle colon. Evidence for single-file ion flow. / Kirk, K. L.; Dawson, D. C.

In: Journal of General Physiology, Vol. 82, No. 3, 1983, p. 297-313.

Research output: Contribution to journalArticle

Kirk, K. L. ; Dawson, D. C. / Basolateral potassium channel in turtle colon. Evidence for single-file ion flow. In: Journal of General Physiology. 1983 ; Vol. 82, No. 3. pp. 297-313.
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