TY - JOUR
T1 - Basolateral potassium channel in turtle colon. Evidence for single-file ion flow
AU - Kirk, K. L.
AU - Dawson, D. C.
PY - 1983
Y1 - 1983
N2 - Treatment of the apical surface of the isolated, ouabain-inhibited turtle colon with the polyene antibiotic amphotericin B permitted the properties of a barium-sensitive potassium conductance in the basolateral membrane to be discerned from the measurements of transepithelial fluxes and electrical currents. Simultaneous measurements of potassium currents and 42K fluxes showed that the movement of potassium was not in accord with simple diffusion. Two other cations, thallium and rubidium, were also permeable and, in addition, exhibited strong interactions with the potassium tracer fluxes. The results indicate that permeant cations exhibit positive coupling, which is consistent with a single-file mechanism of ion translocation through a membrane channel.
AB - Treatment of the apical surface of the isolated, ouabain-inhibited turtle colon with the polyene antibiotic amphotericin B permitted the properties of a barium-sensitive potassium conductance in the basolateral membrane to be discerned from the measurements of transepithelial fluxes and electrical currents. Simultaneous measurements of potassium currents and 42K fluxes showed that the movement of potassium was not in accord with simple diffusion. Two other cations, thallium and rubidium, were also permeable and, in addition, exhibited strong interactions with the potassium tracer fluxes. The results indicate that permeant cations exhibit positive coupling, which is consistent with a single-file mechanism of ion translocation through a membrane channel.
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U2 - 10.1085/jgp.82.3.297
DO - 10.1085/jgp.82.3.297
M3 - Article
C2 - 6313849
AN - SCOPUS:0020605860
SN - 0022-1295
VL - 82
SP - 297
EP - 313
JO - Journal of General Physiology
JF - Journal of General Physiology
IS - 3
ER -