Basolateral potassium channel in turtle colon. Evidence for single-file ion flow

K. L. Kirk, D. C. Dawson

Research output: Contribution to journalArticle

47 Scopus citations

Abstract

Treatment of the apical surface of the isolated, ouabain-inhibited turtle colon with the polyene antibiotic amphotericin B permitted the properties of a barium-sensitive potassium conductance in the basolateral membrane to be discerned from the measurements of transepithelial fluxes and electrical currents. Simultaneous measurements of potassium currents and 42K fluxes showed that the movement of potassium was not in accord with simple diffusion. Two other cations, thallium and rubidium, were also permeable and, in addition, exhibited strong interactions with the potassium tracer fluxes. The results indicate that permeant cations exhibit positive coupling, which is consistent with a single-file mechanism of ion translocation through a membrane channel.

Original languageEnglish (US)
Pages (from-to)297-313
Number of pages17
JournalJournal of General Physiology
Volume82
Issue number3
DOIs
StatePublished - 1983

ASJC Scopus subject areas

  • Physiology

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