TY - JOUR
T1 - Basic protein-specific T-cell lines that induce experimental autoimmune encephalomyelitis in SJL/J mice
T2 - Comparison with lewis rat lines
AU - Bourdette, Dennis N.
AU - Vandenbark, Arthur A.
AU - Meshul, Charles
AU - Whitham, Ruth
AU - Offner, Halina
N1 - Funding Information:
’ This work was supported by the Veterans Administration, the Medical Research Foundation of Oregon, and NIH Grants NS-2322 I and NS-23444. * To whom all correspondence should be addressed at Neurology Service 127, V.A. Medical Center, Portland, OR 97207. 3 Abbreviations used: BP, myelin basic protein; Con A, concanavalin A; cpm, counts per minute; DTH, delayed-type hypersensitivity; EAE, experimental autoimmune encephalomyelitis; PPD, purified protein derivative of mycobacterium; [3H]Tdy, [methyl-3H]thymidine.
PY - 1988/4/1
Y1 - 1988/4/1
N2 - Myelin basic protein (BP)-specific T-cell lines were selected from SJL/J mice using techniques to select similar lines from Lewis rats. SJL/J BP-specific T-cell lines were composed of T cells with the helper/inducer phenotype (Lyt 1.2+, 2.2- and L3T4+) and proliferated in response to both the 1-37 and the 89-169 fragments of guinea pig BP. BP-specific T-cell lines transferred delayed-type hypersensitivity (DTH) responses to BP that persisted for over 60 days. Most recipient animals ( 32 41) developed acute experimental autoimmune encephalomyelitis (EAE), and most survivors ( 19 24) developed chronic relapsing EAE. Spinal cords of animals during both the acute and the chronic phases of illness contained plaques of demyelination and infiltrates of lymphocytes and macrophages. These findings differed from those of Lewis rat BP-specific lines which respond to a different region of BP, transfer DTH responses that last less than 12 days, and induce acute EAE in which demyelination does not occur.
AB - Myelin basic protein (BP)-specific T-cell lines were selected from SJL/J mice using techniques to select similar lines from Lewis rats. SJL/J BP-specific T-cell lines were composed of T cells with the helper/inducer phenotype (Lyt 1.2+, 2.2- and L3T4+) and proliferated in response to both the 1-37 and the 89-169 fragments of guinea pig BP. BP-specific T-cell lines transferred delayed-type hypersensitivity (DTH) responses to BP that persisted for over 60 days. Most recipient animals ( 32 41) developed acute experimental autoimmune encephalomyelitis (EAE), and most survivors ( 19 24) developed chronic relapsing EAE. Spinal cords of animals during both the acute and the chronic phases of illness contained plaques of demyelination and infiltrates of lymphocytes and macrophages. These findings differed from those of Lewis rat BP-specific lines which respond to a different region of BP, transfer DTH responses that last less than 12 days, and induce acute EAE in which demyelination does not occur.
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U2 - 10.1016/0008-8749(88)90304-8
DO - 10.1016/0008-8749(88)90304-8
M3 - Article
C2 - 2451569
AN - SCOPUS:0023886420
SN - 0008-8749
VL - 112
SP - 351
EP - 363
JO - Cellular Immunology
JF - Cellular Immunology
IS - 2
ER -