Basic Fibroblast Growth Factor (bFGF) acts intracellularly to cause the transdifferentiation of avian neural crest-derived Schwann cell precursors into melanocytes

Lawrence (Larry) Sherman, Kate M. Stocker, Richard Morrison, Gary Ciment

Research output: Contribution to journalArticle

110 Citations (Scopus)

Abstract

We previously found that cultured neural crest-derived cells from embryonic quail peripheral nerves, which consist mostly of Schwann cell precursors, gave rise to melanocytes following treatment with basic fibroblast growth factor (bFGF) or 12-O-tetradecanoyl phorbol-13-acetate (TPA). Here, we show that antisense deoxyoligonucleotides targeted against two regions of the bFGF mRNA transcript blocked this TPA-induced transdifferentiation of Schwann cell precursors. Neither sense nor scrambled antisense control oligonucleotides had any effect in this regard. TPA increased bFGF protein expression in cell lysates but not in conditioned media from these cultures, and this expression was localized to the nucleus and cytoplasm. Furthermore, bFGF-neutralizing antibodies and inositolhexakisphosphate (InsP6) both inhibited pigmentation caused by exogenous bFGF, but had no affect on TPA-induced melanogenesis, suggesting that bFGF is not released by these cells. These data indicate that bFGF is necessary for the TPA-induced transdifferentiation of Schwann cell precursors into melanocytes and that bFGF acts via an intracrine mechanism.

Original languageEnglish (US)
Pages (from-to)1313-1326
Number of pages14
JournalDevelopment
Volume118
Issue number4
StatePublished - Aug 1993

Fingerprint

Neural Crest
Melanocytes
Schwann Cells
Fibroblast Growth Factor 2
Tetradecanoylphorbol Acetate
Quail
Antisense Oligonucleotides
Pigmentation
Conditioned Culture Medium
Neutralizing Antibodies
Peripheral Nerves
Cytoplasm
Messenger RNA

Keywords

  • Fibroblast growth factor
  • Intracrine
  • Melanocyte
  • Neural crest
  • Phorbol ester
  • Schwann cell

ASJC Scopus subject areas

  • Cell Biology
  • Anatomy

Cite this

Basic Fibroblast Growth Factor (bFGF) acts intracellularly to cause the transdifferentiation of avian neural crest-derived Schwann cell precursors into melanocytes. / Sherman, Lawrence (Larry); Stocker, Kate M.; Morrison, Richard; Ciment, Gary.

In: Development, Vol. 118, No. 4, 08.1993, p. 1313-1326.

Research output: Contribution to journalArticle

@article{20e8be259dd14c86bc914ae5ebbdcd0a,
title = "Basic Fibroblast Growth Factor (bFGF) acts intracellularly to cause the transdifferentiation of avian neural crest-derived Schwann cell precursors into melanocytes",
abstract = "We previously found that cultured neural crest-derived cells from embryonic quail peripheral nerves, which consist mostly of Schwann cell precursors, gave rise to melanocytes following treatment with basic fibroblast growth factor (bFGF) or 12-O-tetradecanoyl phorbol-13-acetate (TPA). Here, we show that antisense deoxyoligonucleotides targeted against two regions of the bFGF mRNA transcript blocked this TPA-induced transdifferentiation of Schwann cell precursors. Neither sense nor scrambled antisense control oligonucleotides had any effect in this regard. TPA increased bFGF protein expression in cell lysates but not in conditioned media from these cultures, and this expression was localized to the nucleus and cytoplasm. Furthermore, bFGF-neutralizing antibodies and inositolhexakisphosphate (InsP6) both inhibited pigmentation caused by exogenous bFGF, but had no affect on TPA-induced melanogenesis, suggesting that bFGF is not released by these cells. These data indicate that bFGF is necessary for the TPA-induced transdifferentiation of Schwann cell precursors into melanocytes and that bFGF acts via an intracrine mechanism.",
keywords = "Fibroblast growth factor, Intracrine, Melanocyte, Neural crest, Phorbol ester, Schwann cell",
author = "Sherman, {Lawrence (Larry)} and Stocker, {Kate M.} and Richard Morrison and Gary Ciment",
year = "1993",
month = "8",
language = "English (US)",
volume = "118",
pages = "1313--1326",
journal = "Development (Cambridge)",
issn = "0950-1991",
publisher = "Company of Biologists Ltd",
number = "4",

}

TY - JOUR

T1 - Basic Fibroblast Growth Factor (bFGF) acts intracellularly to cause the transdifferentiation of avian neural crest-derived Schwann cell precursors into melanocytes

AU - Sherman, Lawrence (Larry)

AU - Stocker, Kate M.

AU - Morrison, Richard

AU - Ciment, Gary

PY - 1993/8

Y1 - 1993/8

N2 - We previously found that cultured neural crest-derived cells from embryonic quail peripheral nerves, which consist mostly of Schwann cell precursors, gave rise to melanocytes following treatment with basic fibroblast growth factor (bFGF) or 12-O-tetradecanoyl phorbol-13-acetate (TPA). Here, we show that antisense deoxyoligonucleotides targeted against two regions of the bFGF mRNA transcript blocked this TPA-induced transdifferentiation of Schwann cell precursors. Neither sense nor scrambled antisense control oligonucleotides had any effect in this regard. TPA increased bFGF protein expression in cell lysates but not in conditioned media from these cultures, and this expression was localized to the nucleus and cytoplasm. Furthermore, bFGF-neutralizing antibodies and inositolhexakisphosphate (InsP6) both inhibited pigmentation caused by exogenous bFGF, but had no affect on TPA-induced melanogenesis, suggesting that bFGF is not released by these cells. These data indicate that bFGF is necessary for the TPA-induced transdifferentiation of Schwann cell precursors into melanocytes and that bFGF acts via an intracrine mechanism.

AB - We previously found that cultured neural crest-derived cells from embryonic quail peripheral nerves, which consist mostly of Schwann cell precursors, gave rise to melanocytes following treatment with basic fibroblast growth factor (bFGF) or 12-O-tetradecanoyl phorbol-13-acetate (TPA). Here, we show that antisense deoxyoligonucleotides targeted against two regions of the bFGF mRNA transcript blocked this TPA-induced transdifferentiation of Schwann cell precursors. Neither sense nor scrambled antisense control oligonucleotides had any effect in this regard. TPA increased bFGF protein expression in cell lysates but not in conditioned media from these cultures, and this expression was localized to the nucleus and cytoplasm. Furthermore, bFGF-neutralizing antibodies and inositolhexakisphosphate (InsP6) both inhibited pigmentation caused by exogenous bFGF, but had no affect on TPA-induced melanogenesis, suggesting that bFGF is not released by these cells. These data indicate that bFGF is necessary for the TPA-induced transdifferentiation of Schwann cell precursors into melanocytes and that bFGF acts via an intracrine mechanism.

KW - Fibroblast growth factor

KW - Intracrine

KW - Melanocyte

KW - Neural crest

KW - Phorbol ester

KW - Schwann cell

UR - http://www.scopus.com/inward/record.url?scp=0027264597&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027264597&partnerID=8YFLogxK

M3 - Article

C2 - 8269857

AN - SCOPUS:0027264597

VL - 118

SP - 1313

EP - 1326

JO - Development (Cambridge)

JF - Development (Cambridge)

SN - 0950-1991

IS - 4

ER -