Basement membrane diversity detected by monoclonal antibodies

Helena Hessle; Lynn Y. Sakai; David W. Hollister; Robert E. Burgeson; Eva Engvall

doi:10.1111/j.1432-0436.1984.tb01372.x

Basement membrane diversity detected by monoclonal antibodies

Helena Hessle, Lynn Y. Sakai, David W. Hollister, Robert E. Burgeson, Eva Engvall

Molecular and Medical Genetics

Research output: Contribution to journal › Article › peer-review

102 Scopus citations

Abstract

Human fetal membranes or pepsin solubilized proteins thereof were used as immunogens in the production of monoclonal antibodies to basement membrane-associated components. Some of the antibodies obtained reacted with all basement membranes in indirect immunofluorescent-cent microscopy, others reacted with all epithelial but not with endothelial basement membranes, and yet other antibodies reacted only with certain epithelial basement membranes in these tests. The reactivities of the antibodies demonstrate that different basement membranes are (immuno)chemically different and contain unique components in addition to ubiquitous components such as type IV collagen and laminin

Original language	English (US)
Pages (from-to)	49-54
Number of pages	6
Journal	Differentiation
Volume	26
Issue number	1-3
DOIs	https://doi.org/10.1111/j.1432-0436.1984.tb01372.x
State	Published - 1984

ASJC Scopus subject areas

Molecular Biology
Developmental Biology
Cell Biology
Cancer Research

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10.1111/j.1432-0436.1984.tb01372.x

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title = "Basement membrane diversity detected by monoclonal antibodies",

abstract = "Human fetal membranes or pepsin solubilized proteins thereof were used as immunogens in the production of monoclonal antibodies to basement membrane-associated components. Some of the antibodies obtained reacted with all basement membranes in indirect immunofluorescent-cent microscopy, others reacted with all epithelial but not with endothelial basement membranes, and yet other antibodies reacted only with certain epithelial basement membranes in these tests. The reactivities of the antibodies demonstrate that different basement membranes are (immuno)chemically different and contain unique components in addition to ubiquitous components such as type IV collagen and laminin",

author = "Helena Hessle and Sakai, {Lynn Y.} and Hollister, {David W.} and Burgeson, {Robert E.} and Eva Engvall",

note = "Funding Information: Acknowledgements. This work was supported by grants AM 29735 (to DWH), AM 31273, AM 30051, and CA 28896 (to EE), and Cancer Center Support core grant CA 30199 (to the La Jolla Cancer Research Foundation) from Department of Health and Human services.",

year = "1984",

doi = "10.1111/j.1432-0436.1984.tb01372.x",

language = "English (US)",

volume = "26",

pages = "49--54",

journal = "Differentiation",

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TY - JOUR

T1 - Basement membrane diversity detected by monoclonal antibodies

AU - Hessle, Helena

AU - Sakai, Lynn Y.

AU - Hollister, David W.

AU - Burgeson, Robert E.

AU - Engvall, Eva

N1 - Funding Information: Acknowledgements. This work was supported by grants AM 29735 (to DWH), AM 31273, AM 30051, and CA 28896 (to EE), and Cancer Center Support core grant CA 30199 (to the La Jolla Cancer Research Foundation) from Department of Health and Human services.

PY - 1984

Y1 - 1984

N2 - Human fetal membranes or pepsin solubilized proteins thereof were used as immunogens in the production of monoclonal antibodies to basement membrane-associated components. Some of the antibodies obtained reacted with all basement membranes in indirect immunofluorescent-cent microscopy, others reacted with all epithelial but not with endothelial basement membranes, and yet other antibodies reacted only with certain epithelial basement membranes in these tests. The reactivities of the antibodies demonstrate that different basement membranes are (immuno)chemically different and contain unique components in addition to ubiquitous components such as type IV collagen and laminin

AB - Human fetal membranes or pepsin solubilized proteins thereof were used as immunogens in the production of monoclonal antibodies to basement membrane-associated components. Some of the antibodies obtained reacted with all basement membranes in indirect immunofluorescent-cent microscopy, others reacted with all epithelial but not with endothelial basement membranes, and yet other antibodies reacted only with certain epithelial basement membranes in these tests. The reactivities of the antibodies demonstrate that different basement membranes are (immuno)chemically different and contain unique components in addition to ubiquitous components such as type IV collagen and laminin

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Basement membrane diversity detected by monoclonal antibodies

Abstract

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