Babesia duncani as a Model Organism to Study the Development, Virulence, and Drug Susceptibility of Intraerythrocytic Parasites In Vitro and In Vivo

Anasuya C. Pal, Isaline Renard, Pallavi Singh, Pratap Vydyam, Joy E. Chiu, Sovitj Pou, Rolf W. Winter, Rozalia Dodean, Lisa Frueh, Aaron C. Nilsen, Michael K. Riscoe, J. Stone Doggett, Choukri Ben Mamoun

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

Human babesiosis is a malaria-like illness caused by tick-borne intraerythrocytic Babesia parasites of the Apicomplexa phylum. Whereas several species of Babesia can cause severe disease in humans, the ability to propagate Babesia duncani both in vitro in human erythrocytes and in mice makes it a unique pathogen to study Babesia biology and pathogenesis. Here we report an optimized B. duncani in culture-in mouse (ICIM) model that combines continuous in vitro culture of the parasite with a precise model of lethal infection in mice. We demonstrate that B. duncani-infected erythrocytes as well as free merozoites can cause lethal infection in C3H/HeJ mice. Highly reproducible parasitemia and survival outcomes could be established using specific parasite loads in different mouse genetic backgrounds. Using the ICIM model, we discovered 2 new endochin-like quinolone prodrugs (ELQ-331 and ELQ-468) that alone or in combination with atovaquone are highly efficacious against B. duncani and Babesia microti.

Original languageEnglish (US)
Pages (from-to)1267-1275
Number of pages9
JournalThe Journal of infectious diseases
Volume226
Issue number7
DOIs
StatePublished - Sep 28 2022
Externally publishedYes

Keywords

  • Apicomplexa
  • Babesia duncani
  • babesiosis
  • erythrocytes
  • parasite

ASJC Scopus subject areas

  • Immunology and Allergy
  • Infectious Diseases

Fingerprint

Dive into the research topics of 'Babesia duncani as a Model Organism to Study the Development, Virulence, and Drug Susceptibility of Intraerythrocytic Parasites In Vitro and In Vivo'. Together they form a unique fingerprint.

Cite this