TY - JOUR
T1 - B regulatory cells and the tumor-promoting actions of TNF-α during squamous carcinogenesis
AU - Schioppa, Tiziana
AU - Moore, Robert
AU - Thompson, Richard G.
AU - Rosser, Elizabeth C.
AU - Kulbe, Hagen
AU - Nedospasov, Sergei
AU - Mauri, Claudia
AU - Coussens, Lisa M.
AU - Balkwill, Frances R.
PY - 2011/6/28
Y1 - 2011/6/28
N2 - The inflammatory cytokine TNF-α has been recognized as a critical tumor promoter, but the effector cells that mediate its action have not been fully characterized. Because B cells regulate squamous and prostate carcinogenesis, and Tnf -/- mice harbor B-cell defects, we investigated the hypothesis that B cells are important effector cells for TNF-α-mediated promotion of cancer development. Using an adoptive transfer strategy and the 7,12-dimethylbenz[α]anthracene/ terephthalic acid (DMBA/TPA) two-stage model of skin carcinogenesis, we found that both B cells and TNF-α are critical for the development of DMBA/TPA-induced papilloma. Transfer of B cells from DMBA/TPA-treated wild-type mice to Tnf -/- mice rescued papilloma development to a wild-type level, a result not observedwhen B cells from Tnf -/- mice were transferred to Rag2 -/- mice or when TNF-α was eliminated selectively in B cells. Resistance to papilloma developmentin Tnf -/- mice was associated with increased IFN-γ and CD8 + T cells in skin and a significant reduction in IL-10-producing B regulatory cells alongside an increase in IFN-γ-producing CD8 + T cells in the spleen. These data indicate that during DMBA/TPA-induced squamous carcinogenesis TNF-α mediates tumor-promoting activity via regulatory B cells that repress antitumor immunity.
AB - The inflammatory cytokine TNF-α has been recognized as a critical tumor promoter, but the effector cells that mediate its action have not been fully characterized. Because B cells regulate squamous and prostate carcinogenesis, and Tnf -/- mice harbor B-cell defects, we investigated the hypothesis that B cells are important effector cells for TNF-α-mediated promotion of cancer development. Using an adoptive transfer strategy and the 7,12-dimethylbenz[α]anthracene/ terephthalic acid (DMBA/TPA) two-stage model of skin carcinogenesis, we found that both B cells and TNF-α are critical for the development of DMBA/TPA-induced papilloma. Transfer of B cells from DMBA/TPA-treated wild-type mice to Tnf -/- mice rescued papilloma development to a wild-type level, a result not observedwhen B cells from Tnf -/- mice were transferred to Rag2 -/- mice or when TNF-α was eliminated selectively in B cells. Resistance to papilloma developmentin Tnf -/- mice was associated with increased IFN-γ and CD8 + T cells in skin and a significant reduction in IL-10-producing B regulatory cells alongside an increase in IFN-γ-producing CD8 + T cells in the spleen. These data indicate that during DMBA/TPA-induced squamous carcinogenesis TNF-α mediates tumor-promoting activity via regulatory B cells that repress antitumor immunity.
KW - Immune cells
KW - Inflammation
KW - Tumor microenvironment
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U2 - 10.1073/pnas.1100994108
DO - 10.1073/pnas.1100994108
M3 - Article
C2 - 21670304
AN - SCOPUS:79960605654
SN - 0027-8424
VL - 108
SP - 10662
EP - 10667
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 26
ER -